Pixelwise H-score: A novel digital image analysis-based metric to quantify membrane biomarker expression from immunohistochemistry images

Sripad Ram; Pamela Vizcarra; Pamela Whalen; Shibing Deng; C. L. Painter; Amy Jackson-Fisher; Steven Pirie-Shepherd; Xiaoling Xia; Eric L. Powell

doi:10.1371/journal.pone.0245638

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Open Access

Peer-reviewed

Research Article

Pixelwise H-score: A novel digital image analysis-based metric to quantify membrane biomarker expression from immunohistochemistry images

Sripad Ram ,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Project administration, Software, Validation, Visualization, Writing – original draft, Writing – review & editing

* E-mail: Sripad.ram@pfizer.com

Affiliation Drug-Safety Research and Development, Pfizer Inc., San Diego, California, United States of America

https://orcid.org/0000-0002-9770-106X

⨯
Pamela Vizcarra,

Roles Methodology

Affiliation Tumor Morphology Group, Oncology Research and Development, Pfizer Inc., San Diego, California, United States of America
⨯
Pamela Whalen,

Roles Data curation, Methodology, Writing – original draft

Affiliation Tumor Morphology Group, Oncology Research and Development, Pfizer Inc., San Diego, California, United States of America
⨯
Shibing Deng,

Roles Formal analysis

Affiliation Biostatistics Unit, Oncology Research and Development, Pfizer Inc., San Diego, California, United States of America
⨯
C. L. Painter,

Roles Methodology

Affiliation Tumor Morphology Group, Oncology Research and Development, Pfizer Inc., San Diego, California, United States of America
⨯
Amy Jackson-Fisher,

Roles Methodology, Resources

Affiliation Tumor Morphology Group, Oncology Research and Development, Pfizer Inc., San Diego, California, United States of America
⨯
Steven Pirie-Shepherd,

Roles Resources

Affiliation Tumor Morphology Group, Oncology Research and Development, Pfizer Inc., San Diego, California, United States of America
⨯
Xiaoling Xia,

Roles Data curation, Formal analysis

Current address: Ventana Medical Systems, Tucson, Arizona, United States of America

Affiliation Tumor Morphology Group, Oncology Research and Development, Pfizer Inc., San Diego, California, United States of America
⨯
Eric L. Powell

Roles Formal analysis, Methodology, Resources, Writing – original draft, Writing – review & editing

Affiliation Tumor Morphology Group, Oncology Research and Development, Pfizer Inc., San Diego, California, United States of America
⨯

Pixelwise H-score: A novel digital image analysis-based metric to quantify membrane biomarker expression from immunohistochemistry images

Sripad Ram,
Pamela Vizcarra,
Pamela Whalen,
Shibing Deng,
C. L. Painter,
Amy Jackson-Fisher,
Steven Pirie-Shepherd,
Xiaoling Xia,
Eric L. Powell

Published: September 27, 2021
https://doi.org/10.1371/journal.pone.0245638

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Biomarkers
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Cell membranes
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Immunohistochemistry techniques
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Messenger RNA
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Image analysis
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Algorithms
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Tumor resection
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