Efficient multiplexed genome engineering with a polycistronic tRNA and CRISPR guide-RNA reveals an important role of detonator in reproduction of Drosophila melanogaster

Cristin Chon; Grace Chon; Yurika Matsui; Huiqing Zeng; Zhi-Chun Lai; Aimin Liu

doi:10.1371/journal.pone.0245454

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Open Access

Peer-reviewed

Research Article

Efficient multiplexed genome engineering with a polycistronic tRNA and CRISPR guide-RNA reveals an important role of detonator in reproduction of Drosophila melanogaster

Cristin Chon,

Roles Funding acquisition, Investigation

Affiliation Department of Biology, Eberly College of Science, Centers for Cellular Dynamics and Cellular and Molecular Investigation of Neurological Diseases, Huck Institutes of Life Sciences, The Pennsylvania State University, State College, PA, United States of America
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Grace Chon,

Roles Funding acquisition, Investigation

Affiliation Department of Biology, Eberly College of Science, Centers for Cellular Dynamics and Cellular and Molecular Investigation of Neurological Diseases, Huck Institutes of Life Sciences, The Pennsylvania State University, State College, PA, United States of America
⨯
Yurika Matsui,

Roles Investigation, Methodology

Affiliation Department of Biology, Eberly College of Science, Centers for Cellular Dynamics and Cellular and Molecular Investigation of Neurological Diseases, Huck Institutes of Life Sciences, The Pennsylvania State University, State College, PA, United States of America
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Huiqing Zeng,

Roles Investigation, Project administration

Affiliation Department of Biology, Eberly College of Science, Centers for Cellular Dynamics and Cellular and Molecular Investigation of Neurological Diseases, Huck Institutes of Life Sciences, The Pennsylvania State University, State College, PA, United States of America
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Zhi-Chun Lai,

Roles Conceptualization, Supervision, Writing – review & editing

Affiliation Department of Biology, Eberly College of Science, Centers for Cellular Dynamics and Cellular and Molecular Investigation of Neurological Diseases, Huck Institutes of Life Sciences, The Pennsylvania State University, State College, PA, United States of America
⨯
Aimin Liu

Roles Conceptualization, Funding acquisition, Project administration, Supervision, Writing – original draft

* E-mail: AXL25@psu.edu

Affiliation Department of Biology, Eberly College of Science, Centers for Cellular Dynamics and Cellular and Molecular Investigation of Neurological Diseases, Huck Institutes of Life Sciences, The Pennsylvania State University, State College, PA, United States of America

https://orcid.org/0000-0003-0099-0879

⨯

Efficient multiplexed genome engineering with a polycistronic tRNA and CRISPR guide-RNA reveals an important role of detonator in reproduction of Drosophila melanogaster

Cristin Chon,
Grace Chon,
Yurika Matsui,
Huiqing Zeng,
Zhi-Chun Lai,
Aimin Liu

Published: January 14, 2021
https://doi.org/10.1371/journal.pone.0245454

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Eggs
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Drosophila melanogaster
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Polymerase chain reaction
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Invertebrate genomics
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Guide RNA
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