Systemic GFP silencing is associated with high transgene expression in Nicotiana benthamiana

Bill Hendrix; Paul Hoffer; Rick Sanders; Steve Schwartz; Wei Zheng; Brian Eads; Danielle Taylor; Jill Deikman

doi:10.1371/journal.pone.0245422

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Open Access

Peer-reviewed

Research Article

Systemic GFP silencing is associated with high transgene expression in Nicotiana benthamiana

Bill Hendrix ,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Supervision, Validation, Visualization, Writing – original draft

* E-mail: bill.hendrix@bayer.com

Current address: Bayer Crop Science, West Sacramento, California, United States of America

Affiliation Bayer Crop Science, Woodland, California, United States of America

https://orcid.org/0000-0001-8799-1254

⨯
Paul Hoffer,

Roles Data curation, Formal analysis, Investigation, Methodology, Software, Validation, Visualization, Writing – review & editing

Current address: Governor’s Office of Emergency Services, Radiological Preparedness Unit, Mather, California, United States of America

Affiliation Bayer Crop Science, Woodland, California, United States of America

https://orcid.org/0000-0003-3763-8510

⨯
Rick Sanders,

Roles Data curation, Investigation, Methodology, Validation, Writing – review & editing

Affiliation Bayer Crop Science, Woodland, California, United States of America
⨯
Steve Schwartz,

Roles Conceptualization, Investigation, Methodology, Writing – review & editing

Current address: InnerPlant, Davis, California, United States of America

Affiliation Bayer Crop Science, Woodland, California, United States of America
⨯
Wei Zheng,

Roles Conceptualization, Methodology, Writing – review & editing

Current address: Hangzhou Huadi Group Co., Hangzhou, China

Affiliation Bayer Crop Science, Woodland, California, United States of America
⨯
Brian Eads,

Roles Data curation, Formal analysis, Software, Writing – review & editing

Affiliation Bayer Crop Science, Chesterfield Parkway, St. Louis, Missouri, United States of America
⨯
Danielle Taylor,

Roles Data curation, Investigation, Writing – review & editing

Affiliation Bayer Crop Science, Chesterfield Parkway, St. Louis, Missouri, United States of America
⨯
Jill Deikman

Roles Conceptualization, Project administration, Supervision, Writing – review & editing

Affiliation Bayer Crop Science, Woodland, California, United States of America

https://orcid.org/0000-0003-3210-8334

⨯

Systemic GFP silencing is associated with high transgene expression in Nicotiana benthamiana

Bill Hendrix,
Paul Hoffer,
Rick Sanders,
Steve Schwartz,
Wei Zheng,
Brian Eads,
Danielle Taylor,
Jill Deikman

Published: March 15, 2021
https://doi.org/10.1371/journal.pone.0245422

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Leaves
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Green fluorescent protein
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RNA sequencing
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Gene silencing
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Homozygosity
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Transposable elements
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