Development of a reverse transcription recombinase polymerase amplification assay for rapid and direct visual detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)

Yee Ling Lau; Ilyiana binti Ismail; Nur Izati binti Mustapa; Meng Yee Lai; Tuan Suhaila Tuan Soh; Afifah Haji Hassan; Kalaiarasu M. Peariasamy; Yee Leng Lee; Maria Kahar Bador Abdul Kahar; Jennifer Chong; Pik Pin Goh

doi:10.1371/journal.pone.0245164

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Open Access

Peer-reviewed

Research Article

Development of a reverse transcription recombinase polymerase amplification assay for rapid and direct visual detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)

Yee Ling Lau ,

Roles Conceptualization, Data curation, Investigation, Methodology, Project administration, Writing – original draft, Writing – review & editing

* E-mail: lauyeeling@um.edu.my

Affiliation Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia

https://orcid.org/0000-0002-2037-2165

⨯
Ilyiana binti Ismail,

Roles Methodology, Project administration

Affiliation Department of Pathology, Hospital Sungai Buloh, Selangor, Malaysia
⨯
Nur Izati binti Mustapa,

Roles Investigation, Project administration

Affiliation Department of Pathology, Hospital Sungai Buloh, Selangor, Malaysia
⨯
Meng Yee Lai,

Roles Conceptualization, Data curation, Methodology, Project administration

Affiliation Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
⨯
Tuan Suhaila Tuan Soh,

Roles Conceptualization, Project administration

Affiliation Department of Pathology, Hospital Sungai Buloh, Selangor, Malaysia
⨯
Afifah Haji Hassan,

Roles Conceptualization, Project administration

Affiliation Department of Pathology, Hospital Sungai Buloh, Selangor, Malaysia
⨯
Kalaiarasu M. Peariasamy,

Roles Conceptualization, Writing – review & editing

Affiliation Clinical Research Centre, Hospital Sungai Buloh, Selangor, Malaysia
⨯
Yee Leng Lee,

Roles Project administration

Affiliation Clinical Research Centre, Hospital Sungai Buloh, Selangor, Malaysia
⨯
Maria Kahar Bador Abdul Kahar,

Roles Conceptualization, Writing – review & editing

Affiliation Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
⨯
Jennifer Chong,

Roles Investigation, Project administration

Affiliation Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
⨯
Pik Pin Goh

Roles Conceptualization

Affiliation Institute for Clinical Research (ICR), National Institutes of Health (NIH), Ministry of Health Malaysia, Putrajaya, Malaysia
⨯

Development of a reverse transcription recombinase polymerase amplification assay for rapid and direct visual detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)

Yee Ling Lau,
Ilyiana binti Ismail,
Nur Izati binti Mustapa,
Meng Yee Lai,
Tuan Suhaila Tuan Soh,
Afifah Haji Hassan,
Kalaiarasu M. Peariasamy,
Yee Leng Lee,
Maria Kahar Bador Abdul Kahar,
Jennifer Chong

Published: January 6, 2021
https://doi.org/10.1371/journal.pone.0245164

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SARS CoV 2
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Recombinase polymerase amplification
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Reverse transcription
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