Rapid detection of Clostridium perfringens in food by loop-mediated isothermal amplification combined with a lateral flow biosensor

Thanawat Sridapan; Wanida Tangkawsakul; Tavan Janvilisri; Wansika Kiatpathomchai; Sirintip Dangtip; Natharin Ngamwongsatit; Duangjai Nacapricha; Puey Ounjai; Surang Chankhamhaengdecha

doi:10.1371/journal.pone.0245144

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Open Access

Peer-reviewed

Research Article

Rapid detection of Clostridium perfringens in food by loop-mediated isothermal amplification combined with a lateral flow biosensor

Thanawat Sridapan,

Roles Data curation, Formal analysis, Methodology, Validation, Visualization, Writing – original draft

Affiliation Graduate Program in Molecular Medicine, Faculty of Science, Mahidol University, Bangkok, Thailand
⨯
Wanida Tangkawsakul,

Roles Methodology

Affiliation Center of Nanoscience and Nanotechnology, Faculty of Science, Mahidol University, Bangkok, Thailand
⨯
Tavan Janvilisri,

Roles Funding acquisition, Writing – review & editing

Affiliation Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand
⨯
Wansika Kiatpathomchai,

Roles Funding acquisition, Resources

Affiliation Bioengineering and Sensing Technology Research Team, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, Thailand
⨯
Sirintip Dangtip,

Roles Resources

Affiliation Bioengineering and Sensing Technology Research Team, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, Thailand
⨯
Natharin Ngamwongsatit,

Roles Resources

Affiliation Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, Thailand
⨯
Duangjai Nacapricha,

Roles Funding acquisition

Affiliation Department of Chemistry, Faculty of Science, Mahidol University, Bangkok, Thailand
⨯
Puey Ounjai,

Roles Funding acquisition

Affiliation Department of Biology, Faculty of Science, Mahidol University, Bangkok, Thailand

https://orcid.org/0000-0002-3335-6199

⨯
Surang Chankhamhaengdecha

Roles Conceptualization, Formal analysis, Funding acquisition, Investigation, Resources, Supervision, Writing – review & editing

* E-mail: surang.cha@mahidol.ac.th

Affiliation Department of Biology, Faculty of Science, Mahidol University, Bangkok, Thailand

https://orcid.org/0000-0002-5414-4072

⨯

Rapid detection of Clostridium perfringens in food by loop-mediated isothermal amplification combined with a lateral flow biosensor

Thanawat Sridapan,
Wanida Tangkawsakul,
Tavan Janvilisri,
Wansika Kiatpathomchai,
Sirintip Dangtip,
Natharin Ngamwongsatit,
Duangjai Nacapricha,
Puey Ounjai,
Surang Chankhamhaengdecha

Published: January 7, 2021
https://doi.org/10.1371/journal.pone.0245144

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Polymerase chain reaction
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Food
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DNA extraction
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Food poisoning by Clostridium perfringens
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