Genome-wide identification and analysis of highly specific CRISPR/Cas9 editing sites in pepper (Capsicum annuum L.)

Guanliang Li; Ziyan Zhou; Lingrui Liang; Zhao Song; Yafei Hu; Junjie Cui; Weili Chen; Kailin Hu; Jiaowen Cheng

doi:10.1371/journal.pone.0244515

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Open Access

Peer-reviewed

Research Article

Genome-wide identification and analysis of highly specific CRISPR/Cas9 editing sites in pepper (Capsicum annuum L.)

Guanliang Li ,

Contributed equally to this work with: Guanliang Li, Ziyan Zhou

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliations College of Horticulture, South China Agricultural University, Guangzhou, China, South China Key Laboratory of Urban Agriculture, Ministry of Agriculture and Rural Affairs, Guangzhou, China, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, Guangzhou, China
⨯
Ziyan Zhou ,

Contributed equally to this work with: Guanliang Li, Ziyan Zhou

Roles Investigation, Validation, Visualization

Affiliations College of Horticulture, South China Agricultural University, Guangzhou, China, South China Key Laboratory of Urban Agriculture, Ministry of Agriculture and Rural Affairs, Guangzhou, China, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, Guangzhou, China
⨯
Lingrui Liang,

Roles Data curation, Formal analysis, Investigation, Visualization

Affiliations College of Horticulture, South China Agricultural University, Guangzhou, China, South China Key Laboratory of Urban Agriculture, Ministry of Agriculture and Rural Affairs, Guangzhou, China, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, Guangzhou, China
⨯
Zhao Song,

Roles Data curation, Formal analysis

Affiliations College of Horticulture, South China Agricultural University, Guangzhou, China, South China Key Laboratory of Urban Agriculture, Ministry of Agriculture and Rural Affairs, Guangzhou, China, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, Guangzhou, China
⨯
Yafei Hu,

Roles Data curation, Formal analysis

Affiliations College of Horticulture, South China Agricultural University, Guangzhou, China, South China Key Laboratory of Urban Agriculture, Ministry of Agriculture and Rural Affairs, Guangzhou, China, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, Guangzhou, China
⨯
Junjie Cui,

Roles Formal analysis, Investigation

Affiliation Department of Horticulture, College of Food Science and Engineering, Foshan University, Foshan, Guangdong, China
⨯
Weili Chen,

Roles Formal analysis, Investigation

Affiliations College of Horticulture, South China Agricultural University, Guangzhou, China, South China Key Laboratory of Urban Agriculture, Ministry of Agriculture and Rural Affairs, Guangzhou, China, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, Guangzhou, China
⨯
Kailin Hu,

Roles Conceptualization, Funding acquisition, Methodology, Supervision

Affiliations College of Horticulture, South China Agricultural University, Guangzhou, China, South China Key Laboratory of Urban Agriculture, Ministry of Agriculture and Rural Affairs, Guangzhou, China, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, Guangzhou, China
⨯
Jiaowen Cheng

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Visualization, Writing – original draft, Writing – review & editing

* E-mail: jiaolong1015@126.com

Affiliations College of Horticulture, South China Agricultural University, Guangzhou, China, South China Key Laboratory of Urban Agriculture, Ministry of Agriculture and Rural Affairs, Guangzhou, China, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, Guangzhou, China

https://orcid.org/0000-0002-7004-5138

⨯

Genome-wide identification and analysis of highly specific CRISPR/Cas9 editing sites in pepper (Capsicum annuum L.)

Guanliang Li,
Ziyan Zhou,
Lingrui Liang,
Zhao Song,
Yafei Hu,
Junjie Cui,
Weili Chen,
Kailin Hu,
Jiaowen Cheng

Published: December 29, 2020
https://doi.org/10.1371/journal.pone.0244515

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Genomics
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CRISPR
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Polymerase chain reaction
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Sequence alignment
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Insertion mutation
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Genome analysis
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