A single-molecule counting approach for convenient and ultrasensitive measurement of restriction digest efficiencies

Yi Zhang; Takuro Nunoura; Daisuke Nishiura; Miho Hirai; Shigeru Shimamura; Kanako Kurosawa; Chieko Ishiwata; Shigeru Deguchi

doi:10.1371/journal.pone.0244464

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Open Access

Peer-reviewed

Research Article

A single-molecule counting approach for convenient and ultrasensitive measurement of restriction digest efficiencies

Yi Zhang ,

Roles Conceptualization, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Validation, Visualization, Writing – original draft, Writing – review & editing

* E-mail: zhangyi@jamstec.go.jp

Affiliation SUGAR Program, X-star, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan

https://orcid.org/0000-0002-1886-737X

⨯
Takuro Nunoura,

Roles Formal analysis, Resources, Supervision, Writing – review & editing

Affiliation Research Center for Bioscience and Nanoscience, Research Institute for Marine Resources Utilization, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan
⨯
Daisuke Nishiura,

Roles Data curation, Formal analysis, Resources, Software, Visualization

Affiliation Center for Mathematical Science and Advanced Technology, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan
⨯
Miho Hirai,

Roles Investigation, Validation

Affiliation SUGAR Program, X-star, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan
⨯
Shigeru Shimamura,

Roles Investigation

Affiliation SUGAR Program, X-star, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan
⨯
Kanako Kurosawa,

Roles Investigation, Resources, Writing – review & editing

Affiliation SUGAR Program, X-star, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan
⨯
Chieko Ishiwata,

Roles Data curation, Software

Affiliation Center for Mathematical Science and Advanced Technology, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan
⨯
Shigeru Deguchi

Roles Resources, Supervision, Writing – review & editing

Affiliation Research Center for Bioscience and Nanoscience, Research Institute for Marine Resources Utilization, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan
⨯

A single-molecule counting approach for convenient and ultrasensitive measurement of restriction digest efficiencies

Yi Zhang,
Takuro Nunoura,
Daisuke Nishiura,
Miho Hirai,
Shigeru Shimamura,
Kanako Kurosawa,
Chieko Ishiwata,
Shigeru Deguchi

Published: December 31, 2020
https://doi.org/10.1371/journal.pone.0244464

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DNA cleavage
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