Optimised generation of iPSC-derived macrophages and dendritic cells that are functionally and transcriptionally similar to their primary counterparts

Susan Monkley; Jayendra Kumar Krishnaswamy; Melker Göransson; Maryam Clausen; Johan Meuller; Kristofer Thörn; Ryan Hicks; Stephen Delaney; Louise Stjernborg

doi:10.1371/journal.pone.0243807

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Open Access

Peer-reviewed

Research Article

Optimised generation of iPSC-derived macrophages and dendritic cells that are functionally and transcriptionally similar to their primary counterparts

Susan Monkley ,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Software, Visualization, Writing – original draft, Writing – review & editing

* E-mail: susan.monkley1@astrazeneca.com

Affiliation Translational Science and Experimental Medicine, Research and Early Development, Respiratory and Immunology (R&I), BioPharmaceuticals R&D, AstraZeneca, Gothenburg, Sweden

https://orcid.org/0000-0001-9174-6278

⨯
Jayendra Kumar Krishnaswamy,

Roles Conceptualization, Formal analysis, Investigation, Methodology, Writing – original draft, Writing – review & editing

Current address: Galderma Pharma SA, Lausanne, Switzerland

Affiliation Bioscience, Research and Early Development, Respiratory and Immunology (R&I), BioPharmaceuticals R&D, AstraZeneca, Gothenburg, Sweden
⨯
Melker Göransson,

Roles Conceptualization, Formal analysis, Investigation, Writing – review & editing

Affiliation Bioscience, Research and Early Development, Respiratory and Immunology (R&I), BioPharmaceuticals R&D, AstraZeneca, Gothenburg, Sweden
⨯
Maryam Clausen,

Roles Conceptualization, Methodology, Writing – review & editing

Affiliation Discovery Biology, Discovery Sciences, BioPharmaceuticals R&D, AstraZeneca, Gothenburg, Sweden
⨯
Johan Meuller,

Roles Conceptualization, Formal analysis, Investigation, Writing – review & editing

Current address: Mölnlycke Healthcare, Gothenburg, Sweden

Affiliation Discovery Biology, Discovery Sciences, BioPharmaceuticals R&D, AstraZeneca, Gothenburg, Sweden
⨯
Kristofer Thörn,

Roles Conceptualization, Investigation, Writing – review & editing

Affiliation Translational Science and Experimental Medicine, Research and Early Development, Respiratory and Immunology (R&I), BioPharmaceuticals R&D, AstraZeneca, Gothenburg, Sweden
⨯
Ryan Hicks,

Roles Conceptualization, Formal analysis

Affiliation BioPharmaceuticals R&D Cell Therapy, Research and Early Development, Cardiovascular, Renal and Metabolism (CVRM), BioPharmaceuticals R&D, AstraZeneca, Gothenburg, Sweden
⨯
Stephen Delaney,

Roles Conceptualization, Formal analysis, Writing – original draft, Writing – review & editing

Affiliation Bioscience, Research and Early Development, Respiratory and Immunology (R&I), BioPharmaceuticals R&D, AstraZeneca, Gothenburg, Sweden
⨯
Louise Stjernborg

Roles Conceptualization, Formal analysis, Investigation, Methodology, Project administration, Writing – original draft, Writing – review & editing

Affiliation Discovery Biology, Discovery Sciences, BioPharmaceuticals R&D, AstraZeneca, Gothenburg, Sweden
⨯

Optimised generation of iPSC-derived macrophages and dendritic cells that are functionally and transcriptionally similar to their primary counterparts

Susan Monkley,
Jayendra Kumar Krishnaswamy,
Melker Göransson,
Maryam Clausen,
Johan Meuller,
Kristofer Thörn,
Ryan Hicks,
Stephen Delaney,
Louise Stjernborg

Published: December 17, 2020
https://doi.org/10.1371/journal.pone.0243807

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