Scanning single-molecule counting system for Eprobe with highly simple and effective approach

Takeshi Hanami; Tetsuya Tanabe; Takuya Hanashi; Mitsushiro Yamaguchi; Hidetaka Nakata; Yasumasa Mitani; Yasumasa Kimura; Takahiro Soma; Kengo Usui; Michiko Isobe; Takashi Ogawa; Masayoshi Itoh; Yoshihide Hayashizaki; Seiji Kondo

doi:10.1371/journal.pone.0243319

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Open Access

Peer-reviewed

Research Article

Scanning single-molecule counting system for Eprobe with highly simple and effective approach

Takeshi Hanami ,

Roles Conceptualization, Data curation, Investigation, Methodology, Project administration, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

* E-mail: thanami@riken.jp (TH); te_tanabe@ot.olympus.co.jp (TT)

Affiliations Genetic Diagnosis Technology Unit, RIKEN Center for Integrative Medical Science, Yokohama, Kanagawa, Japan, RIKEN Innovation Center, Wako, Saitama, Japan

https://orcid.org/0000-0003-1868-1409

⨯
Tetsuya Tanabe ,

Roles Conceptualization, Data curation, Formal analysis, Project administration, Software, Supervision, Validation, Visualization, Writing – original draft

* E-mail: thanami@riken.jp (TH); te_tanabe@ot.olympus.co.jp (TT)

Affiliations Genetic Diagnosis Technology Unit, RIKEN Center for Integrative Medical Science, Yokohama, Kanagawa, Japan, RIKEN Innovation Center, Wako, Saitama, Japan, Advanced Analysis Technology Dept., Medical Technology R&D Division, Olympus Corporation, Hachioji, Tokyo, Japan
⨯
Takuya Hanashi,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Software, Supervision, Validation, Visualization, Writing – review & editing

Affiliations Genetic Diagnosis Technology Unit, RIKEN Center for Integrative Medical Science, Yokohama, Kanagawa, Japan, RIKEN Innovation Center, Wako, Saitama, Japan, Advanced Analysis Technology Dept., Medical Technology R&D Division, Olympus Corporation, Hachioji, Tokyo, Japan
⨯
Mitsushiro Yamaguchi,

Roles Conceptualization, Data curation, Formal analysis, Resources, Software, Supervision, Validation, Visualization

Affiliations Genetic Diagnosis Technology Unit, RIKEN Center for Integrative Medical Science, Yokohama, Kanagawa, Japan, RIKEN Innovation Center, Wako, Saitama, Japan, Advanced Analysis Technology Dept., Medical Technology R&D Division, Olympus Corporation, Hachioji, Tokyo, Japan
⨯
Hidetaka Nakata,

Roles Conceptualization, Data curation, Formal analysis, Software, Validation, Visualization

Affiliations RIKEN Innovation Center, Wako, Saitama, Japan, Advanced Analysis Technology Dept., Medical Technology R&D Division, Olympus Corporation, Hachioji, Tokyo, Japan
⨯
Yasumasa Mitani,

Roles Conceptualization, Supervision, Validation

Affiliation K.K. DNAFORM, Yokohama, Kanagawa, Japan
⨯
Yasumasa Kimura,

Roles Conceptualization, Data curation, Validation

Affiliation K.K. DNAFORM, Yokohama, Kanagawa, Japan
⨯
Takahiro Soma,

Roles Methodology, Resources, Writing – review & editing

Affiliation Genetic Diagnosis Technology Unit, RIKEN Center for Integrative Medical Science, Yokohama, Kanagawa, Japan
⨯
Kengo Usui,

Roles Funding acquisition, Methodology, Resources, Supervision, Writing – review & editing

Affiliation Genetic Diagnosis Technology Unit, RIKEN Center for Integrative Medical Science, Yokohama, Kanagawa, Japan
⨯
Michiko Isobe,

Roles Data curation, Formal analysis, Methodology, Validation

Affiliation RIKEN Innovation Center, Wako, Saitama, Japan
⨯
Takashi Ogawa,

Roles Data curation, Formal analysis, Resources, Validation

Affiliation RIKEN Innovation Center, Wako, Saitama, Japan
⨯
Masayoshi Itoh,

Roles Conceptualization, Data curation, Methodology, Validation

Affiliations Genetic Diagnosis Technology Unit, RIKEN Center for Integrative Medical Science, Yokohama, Kanagawa, Japan, RIKEN Innovation Center, Wako, Saitama, Japan, RIKEN Preventive Medicine and Diagnosis Innovation Program, Wako, Saitama, Japan

https://orcid.org/0000-0002-1772-318X

⨯
Yoshihide Hayashizaki,

Roles Conceptualization, Funding acquisition, Methodology, Writing – review & editing

Affiliation RIKEN Preventive Medicine and Diagnosis Innovation Program, Wako, Saitama, Japan
⨯
Seiji Kondo

Roles Conceptualization, Funding acquisition, Investigation, Methodology, Project administration, Supervision, Validation

Affiliations RIKEN Innovation Center, Wako, Saitama, Japan, Advanced Analysis Technology Dept., Medical Technology R&D Division, Olympus Corporation, Hachioji, Tokyo, Japan
⨯

Scanning single-molecule counting system for Eprobe with highly simple and effective approach

Takeshi Hanami,
Tetsuya Tanabe,
Takuya Hanashi,
Mitsushiro Yamaguchi,
Hidetaka Nakata,
Yasumasa Mitani,
Yasumasa Kimura,
Takahiro Soma,
Kengo Usui,
Michiko Isobe

Published: December 15, 2020
https://doi.org/10.1371/journal.pone.0243319

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