Bacterial genome editing by coupling Cre-lox and CRISPR-Cas9 systems

Hualan Liu; David S. Robinson; Zong-Yen Wu; Rita Kuo; Yasuo Yoshikuni; Ian K. Blaby; Jan-Fang Cheng

doi:10.1371/journal.pone.0241867

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Open Access

Peer-reviewed

Research Article

Bacterial genome editing by coupling Cre-lox and CRISPR-Cas9 systems

Hualan Liu ,

Contributed equally to this work with: Hualan Liu, David S. Robinson

Roles Conceptualization, Writing – original draft

Affiliations US Department of Energy Joint Genome Institute, Berkeley, California, United States of America, Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America

https://orcid.org/0000-0002-9636-3855

⨯
David S. Robinson ,

Contributed equally to this work with: Hualan Liu, David S. Robinson

Roles Investigation, Validation

Affiliation US Department of Energy Joint Genome Institute, Berkeley, California, United States of America
⨯
Zong-Yen Wu,

Roles Investigation, Validation

Affiliations US Department of Energy Joint Genome Institute, Berkeley, California, United States of America, Department of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan, ROC
⨯
Rita Kuo,

Roles Investigation, Validation

Affiliation US Department of Energy Joint Genome Institute, Berkeley, California, United States of America
⨯
Yasuo Yoshikuni,

Roles Conceptualization

Affiliations US Department of Energy Joint Genome Institute, Berkeley, California, United States of America, Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America, Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America
⨯
Ian K. Blaby,

Roles Conceptualization, Writing – review & editing

Affiliations US Department of Energy Joint Genome Institute, Berkeley, California, United States of America, Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America
⨯
Jan-Fang Cheng

Roles Conceptualization, Methodology, Supervision, Writing – review & editing

* E-mail: jfcheng@lbl.gov

Affiliations US Department of Energy Joint Genome Institute, Berkeley, California, United States of America, Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America
⨯

Bacterial genome editing by coupling Cre-lox and CRISPR-Cas9 systems

Hualan Liu,
David S. Robinson,
Zong-Yen Wu,
Rita Kuo,
Yasuo Yoshikuni,
Ian K. Blaby,
Jan-Fang Cheng

Published: November 4, 2020
https://doi.org/10.1371/journal.pone.0241867

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Polymerase chain reaction
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CRISPR
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Genomics
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DNA repair
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Bacterial genomics
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Chromosomal deletions
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Transposable elements
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Plasmid construction
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