RNase H-dependent PCR enables highly specific amplification of antibody variable domains from single B-cells

John Crissman; Yuhao Lin; Kevin Separa; Madeleine Duquette; Michael Cohen; Candyd Velasquez; Thomas Cujec

doi:10.1371/journal.pone.0241803

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Open Access

Peer-reviewed

Research Article

RNase H-dependent PCR enables highly specific amplification of antibody variable domains from single B-cells

John Crissman,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing – original draft, Writing – review & editing

Affiliation Department of Biologics Automation and High-Throughput Technologies, Eli Lilly and Company Biotechnology Center, San Diego, California, United States of America
⨯
Yuhao Lin,

Roles Data curation, Formal analysis, Investigation, Methodology, Writing – original draft

Affiliation Department of Biologics IT, Eli Lilly and Company Biotechnology Center, San Diego, California, United States of America
⨯
Kevin Separa,

Roles Data curation, Investigation, Writing – original draft

Affiliation Department of Biologics Automation and High-Throughput Technologies, Eli Lilly and Company Biotechnology Center, San Diego, California, United States of America
⨯
Madeleine Duquette,

Roles Data curation, Investigation, Writing – review & editing

Affiliation Department of Biologics Automation and High-Throughput Technologies, Eli Lilly and Company Biotechnology Center, San Diego, California, United States of America
⨯
Michael Cohen,

Roles Data curation, Investigation

Affiliation Department of Biologics Automation and High-Throughput Technologies, Eli Lilly and Company Biotechnology Center, San Diego, California, United States of America
⨯
Candyd Velasquez,

Roles Data curation, Investigation

Affiliation Department of Biologics Automation and High-Throughput Technologies, Eli Lilly and Company Biotechnology Center, San Diego, California, United States of America
⨯
Thomas Cujec

Roles Conceptualization, Formal analysis, Investigation, Methodology, Supervision, Writing – original draft, Writing – review & editing

* E-mail: cujecth@lilly.com

Affiliation Department of Biologics Automation and High-Throughput Technologies, Eli Lilly and Company Biotechnology Center, San Diego, California, United States of America

https://orcid.org/0000-0002-6582-7848

⨯

RNase H-dependent PCR enables highly specific amplification of antibody variable domains from single B-cells

John Crissman,
Yuhao Lin,
Kevin Separa,
Madeleine Duquette,
Michael Cohen,
Candyd Velasquez,
Thomas Cujec

Published: November 5, 2020
https://doi.org/10.1371/journal.pone.0241803

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Polymerase chain reaction
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Antibodies
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B cells
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Cloning
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Signal amplification
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Enzyme-linked immunoassays
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Ribonucleases
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Next-generation sequencing
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