Expression, purification and characterization of α-synuclein fibrillar specific scFv from inclusion bodies

Vijay Gupta; Indulekha P. Sudhakaran; Zeyaul Islam; Nishant N. Vaikath; Issam Hmila; Tamas Lukacsovich; Prasanna R. Kolatkar; Omar M. A. El-Agnaf

doi:10.1371/journal.pone.0241773

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Open Access

Peer-reviewed

Research Article

Expression, purification and characterization of α-synuclein fibrillar specific scFv from inclusion bodies

Vijay Gupta,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliation Neurological Disorder Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation, Doha, Qatar
⨯
Indulekha P. Sudhakaran,

Roles Data curation, Formal analysis, Investigation, Methodology, Validation, Writing – original draft

Affiliation Neurological Disorder Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation, Doha, Qatar
⨯
Zeyaul Islam,

Roles Data curation, Formal analysis, Investigation, Methodology, Software, Validation, Visualization, Writing – original draft

Affiliation Diabetes Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation, Doha, Qatar

https://orcid.org/0000-0002-5444-3910

⨯
Nishant N. Vaikath,

Roles Data curation, Formal analysis, Investigation, Methodology, Validation, Writing – original draft

Affiliation Neurological Disorder Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation, Doha, Qatar
⨯
Issam Hmila,

Roles Data curation, Formal analysis, Investigation, Methodology, Validation, Writing – original draft

Affiliation Neurological Disorder Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation, Doha, Qatar
⨯
Tamas Lukacsovich,

Roles Data curation, Formal analysis, Investigation, Methodology, Validation

Affiliation Brain Research Institute, University of Zürich, Zürich, Switzerland
⨯
Prasanna R. Kolatkar,

Roles Formal analysis, Supervision, Writing – review & editing

Affiliation Diabetes Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation, Doha, Qatar
⨯
Omar M. A. El-Agnaf

Roles Conceptualization, Funding acquisition, Project administration, Supervision, Writing – review & editing

* E-mail: oelagnaf@hbku.edu.qa

Affiliation Neurological Disorder Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation, Doha, Qatar

https://orcid.org/0000-0002-6850-8084

⨯

Expression, purification and characterization of α-synuclein fibrillar specific scFv from inclusion bodies

Vijay Gupta,
Indulekha P. Sudhakaran,
Zeyaul Islam,
Nishant N. Vaikath,
Issam Hmila,
Tamas Lukacsovich,
Prasanna R. Kolatkar,
Omar M. A. El-Agnaf

Published: November 6, 2020
https://doi.org/10.1371/journal.pone.0241773

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