Detection of KRAS mutations in liquid biopsies from metastatic colorectal cancer patients using droplet digital PCR, Idylla, and next generation sequencing

• Matilda Holm ,

  Contributed equally to this work with: Matilda Holm, Emma Andersson

  Roles Data curation, Formal analysis, Investigation, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: matilda.holm@helsinki.fi

  Affiliations Department of Pathology, Medicum, Faculty of Medicine, University of Helsinki and HUSLAB, HUS Diagnostic Center, Helsinki University Hospital, Helsinki, Finland, Applied Tumor Genomics Research Program, Research Programs Unit, Faculty of Medicine, University of Helsinki, Helsinki, Finland, Department of Surgery, Medicum, Faculty of Medicine, University of Helsinki and Helsinki University Hospital, Helsinki, Finland, Translational Cancer Medicine Research Program, Research Programs Unit, Faculty of Medicine, University of Helsinki, Helsinki, Finland

  https://orcid.org/0000-0001-8453-3936

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• Emma Andersson ,

  Contributed equally to this work with: Matilda Holm, Emma Andersson

  Roles Methodology, Writing – original draft, Writing – review & editing

  Affiliation Department of Genetics, HUSLAB, HUS Diagnostic Center, Helsinki University Hospital and University of Helsinki, Helsinki, Finland

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• Emerik Osterlund,

  Roles Data curation, Investigation, Writing – review & editing

  Affiliations Department of Surgery, Medicum, Faculty of Medicine, University of Helsinki and Helsinki University Hospital, Helsinki, Finland, Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden

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• Ali Ovissi,

  Roles Investigation, Methodology, Writing – review & editing

  Affiliation Department of Radiology, HUS Diagnostic Center, Helsinki University Hospital, and University of Helsinki, Helsinki, Finland

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• Leena-Maija Soveri,

  Roles Conceptualization, Investigation, Writing – review & editing

  Affiliations Department of Oncology, Clinicum, Helsinki University Hospital and University of Helsinki, Helsinki, Finland, Hyvinkää Hospital and Hyvinkää Homecare, Hyvinkää, Finland

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• Anna-Kaisa Anttonen,

  Roles Resources, Writing – review & editing

  Affiliation Department of Genetics, HUSLAB, HUS Diagnostic Center, Helsinki University Hospital and University of Helsinki, Helsinki, Finland

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• Soili Kytölä,

  Roles Data curation, Methodology, Supervision, Validation, Writing – review & editing

  Affiliation Department of Genetics, HUSLAB, HUS Diagnostic Center, Helsinki University Hospital and University of Helsinki, Helsinki, Finland

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• Kristiina Aittomäki,

  Roles Conceptualization, Funding acquisition, Resources, Writing – review & editing

  Affiliation Department of Genetics, HUSLAB, HUS Diagnostic Center, Helsinki University Hospital and University of Helsinki, Helsinki, Finland

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• Pia Osterlund ,

  Roles Conceptualization, Data curation, Funding acquisition, Resources, Supervision, Writing – review & editing

  ¶‡ PO and AR authors also contributed equally to this work as the last authors.

  Affiliations Department of Oncology, Clinicum, Helsinki University Hospital and University of Helsinki, Helsinki, Finland, Department of Oncology, Tampere University Hospital, Tampere, Finland, Faculty of Medicine and Life Sciences, Tampere University, Tampere, Finland

  https://orcid.org/0000-0002-7124-3515

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• Ari Ristimäki

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Project administration, Resources, Supervision, Writing – review & editing

  ¶‡ PO and AR authors also contributed equally to this work as the last authors.

  Affiliations Department of Pathology, Medicum, Faculty of Medicine, University of Helsinki and HUSLAB, HUS Diagnostic Center, Helsinki University Hospital, Helsinki, Finland, Applied Tumor Genomics Research Program, Research Programs Unit, Faculty of Medicine, University of Helsinki, Helsinki, Finland

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