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Open Access
Peer-reviewed
Research Article
Rapid SARS-CoV-2 testing in primary material based on a novel multiplex RT-LAMP assay
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Bernhard Schermer ,
Roles Conceptualization, Funding acquisition, Investigation, Project administration, Writing – original draft, Writing – review & editing
* E-mail: bernhard.schermer@uk-koeln.de
Affiliations Department II of Internal Medicine, University of Cologne, Faculty of Medicine and University Hospital Cologne, Cologne, Germany, Center for Molecular Medicine Cologne, University of Cologne, Faculty of Medicine and University Hospital Cologne, Cologne, Germany, Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, Germany
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Francesca Fabretti,
Roles Investigation, Methodology, Validation, Writing – review & editing
Affiliations Department II of Internal Medicine, University of Cologne, Faculty of Medicine and University Hospital Cologne, Cologne, Germany, Center for Molecular Medicine Cologne, University of Cologne, Faculty of Medicine and University Hospital Cologne, Cologne, Germany, Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, Germany
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Maximilian Damagnez,
Roles Investigation, Writing – review & editing
Affiliation Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of Cologne, Cologne, Germany
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Veronica Di Cristanziano,
Roles Methodology, Resources, Validation, Writing – review & editing
Affiliation Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of Cologne, Cologne, Germany
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Eva Heger,
Roles Resources, Validation, Writing – review & editing
Affiliation Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of Cologne, Cologne, Germany
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Sita Arjune,
Roles Data curation, Methodology, Resources
Affiliations Department II of Internal Medicine, University of Cologne, Faculty of Medicine and University Hospital Cologne, Cologne, Germany, Center for Molecular Medicine Cologne, University of Cologne, Faculty of Medicine and University Hospital Cologne, Cologne, Germany, Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, Germany
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Nathan A. Tanner,
Roles Methodology, Writing – review & editing
Affiliation New England Biolabs, Ipswich, MA, United States of America
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Thomas Imhof,
Roles Resources
Affiliations Center for Molecular Medicine Cologne, University of Cologne, Faculty of Medicine and University Hospital Cologne, Cologne, Germany, Institute for Dental Research and Oral Musculoskeletal Biology, Center for Biochemistry, University of Cologne, Cologne, Germany
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Manuel Koch,
Roles Resources, Writing – review & editing
Affiliations Center for Molecular Medicine Cologne, University of Cologne, Faculty of Medicine and University Hospital Cologne, Cologne, Germany, Institute for Dental Research and Oral Musculoskeletal Biology, Center for Biochemistry, University of Cologne, Cologne, Germany
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Alim Ladha,
Roles Resources
Affiliations Howard Hughes Medical Institute, Cambridge, MA, United States of America, Broad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, MA, United States of America, McGovern Institute for Brain Research at Massachusetts Institute of Technology, Cambridge, MA, United States of America, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, United States of America
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Julia Joung,
Roles Resources
Affiliations Howard Hughes Medical Institute, Cambridge, MA, United States of America, Broad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, MA, United States of America, McGovern Institute for Brain Research at Massachusetts Institute of Technology, Cambridge, MA, United States of America, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, United States of America
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Jonathan S. Gootenberg,
Roles Resources
Affiliations McGovern Institute for Brain Research at Massachusetts Institute of Technology, Cambridge, MA, United States of America, Massachusetts Consortium for Pathogen Readiness, Boston, MA, United States of America
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Omar O. Abudayyeh,
Roles Methodology, Resources
Affiliations McGovern Institute for Brain Research at Massachusetts Institute of Technology, Cambridge, MA, United States of America, Massachusetts Consortium for Pathogen Readiness, Boston, MA, United States of America
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Volker Burst,
Roles Conceptualization, Funding acquisition, Methodology, Resources, Writing – review & editing
Affiliations Department II of Internal Medicine, University of Cologne, Faculty of Medicine and University Hospital Cologne, Cologne, Germany, Emergency Department, Faculty of Medicine and University Hospital Cologne, Cologne, Germany
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Feng Zhang,
Roles Methodology, Resources
Affiliations Howard Hughes Medical Institute, Cambridge, MA, United States of America, Broad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, MA, United States of America, McGovern Institute for Brain Research at Massachusetts Institute of Technology, Cambridge, MA, United States of America, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, United States of America, Massachusetts Consortium for Pathogen Readiness, Boston, MA, United States of America, Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, United States of America
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Florian Klein,
Roles Conceptualization, Funding acquisition, Methodology, Supervision, Writing – review & editing
Affiliations Center for Molecular Medicine Cologne, University of Cologne, Faculty of Medicine and University Hospital Cologne, Cologne, Germany, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of Cologne, Cologne, Germany, German Center for Infection Research, Partner Site Bonn-Cologne, Cologne, Germany
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Thomas Benzing,
Roles Conceptualization, Writing – review & editing
Affiliations Department II of Internal Medicine, University of Cologne, Faculty of Medicine and University Hospital Cologne, Cologne, Germany, Center for Molecular Medicine Cologne, University of Cologne, Faculty of Medicine and University Hospital Cologne, Cologne, Germany, Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, Germany
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Roman-Ulrich Müller
Roles Conceptualization, Funding acquisition, Supervision, Writing – original draft, Writing – review & editing
Affiliations Department II of Internal Medicine, University of Cologne, Faculty of Medicine and University Hospital Cologne, Cologne, Germany, Center for Molecular Medicine Cologne, University of Cologne, Faculty of Medicine and University Hospital Cologne, Cologne, Germany, Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, Germany
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Rapid SARS-CoV-2 testing in primary material based on a novel multiplex RT-LAMP assay
- Bernhard Schermer,
- Francesca Fabretti,
- Maximilian Damagnez,
- Veronica Di Cristanziano,
- Eva Heger,
- Sita Arjune,
- Nathan A. Tanner,
- Thomas Imhof,
- Manuel Koch,
- Alim Ladha
- Published: November 2, 2020
- https://doi.org/10.1371/journal.pone.0238612