Enabling one-pot Golden Gate assemblies of unprecedented complexity using data-optimized assembly design

• John M. Pryor ,

  Contributed equally to this work with: John M. Pryor, Vladimir Potapov

  Roles Conceptualization, Investigation, Methodology, Visualization, Writing – original draft, Writing – review & editing

  Affiliation Research Department, New England Biolabs, Ipswich, Massachusetts, United States of America

  http://orcid.org/0000-0001-7000-9383

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• Vladimir Potapov ,

  Contributed equally to this work with: John M. Pryor, Vladimir Potapov

  Roles Conceptualization, Formal analysis, Methodology, Software, Visualization, Writing – original draft, Writing – review & editing

  Affiliation Research Department, New England Biolabs, Ipswich, Massachusetts, United States of America

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• Rebecca B. Kucera,

  Roles Conceptualization, Methodology, Resources, Writing – review & editing

  Affiliation Applications and Product Development, New England Biolabs, Ipswich, Massachusetts, United States of America

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• Katharina Bilotti,

  Roles Conceptualization, Visualization, Writing – original draft, Writing – review & editing

  Affiliation Research Department, New England Biolabs, Ipswich, Massachusetts, United States of America

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• Eric J. Cantor,

  Roles Conceptualization, Methodology, Supervision, Writing – review & editing

  Affiliation Applications and Product Development, New England Biolabs, Ipswich, Massachusetts, United States of America

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• Gregory J. S. Lohman

  Roles Conceptualization, Methodology, Supervision, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: Lohman@neb.com

  Affiliation Research Department, New England Biolabs, Ipswich, Massachusetts, United States of America

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