Characterization of CRISPR/Cas9 RANKL knockout mesenchymal stem cell clones based on single-cell printing technology and Emulsion Coupling assay as a low-cellularity workflow for single-cell cloning

• Tobias Gross ,

  Roles Conceptualization, Data curation, Investigation, Methodology, Project administration, Software, Validation, Visualization, Writing – original draft

  * E-mail: tobias.gross@imtek.uni-freiburg.de

  Affiliation Laboratory for MEMS Applications, IMTEK - Department of Microsystems Engineering, University of Freiburg, Freiburg, Germany

  https://orcid.org/0000-0002-8081-2652

  ⨯
• Csaba Jeney,

  Roles Conceptualization, Formal analysis, Methodology, Software, Supervision, Validation, Writing – review & editing

  Affiliation Laboratory for MEMS Applications, IMTEK - Department of Microsystems Engineering, University of Freiburg, Freiburg, Germany

  ⨯
• Darius Halm,

  Roles Methodology

  Affiliation Department of Plastic and Hand Surgery, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany

  ⨯
• Günter Finkenzeller,

  Roles Conceptualization

  Affiliation Department of Plastic and Hand Surgery, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany

  ⨯
• G. Björn Stark,

  Roles Resources

  Affiliation Department of Plastic and Hand Surgery, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany

  ⨯
• Roland Zengerle,

  Roles Resources

  Affiliations Laboratory for MEMS Applications, IMTEK - Department of Microsystems Engineering, University of Freiburg, Freiburg, Germany, Hahn-Schickard, Freiburg, Germany

  ⨯
• Peter Koltay,

  Roles Conceptualization, Funding acquisition, Resources

  Affiliations Laboratory for MEMS Applications, IMTEK - Department of Microsystems Engineering, University of Freiburg, Freiburg, Germany, Hahn-Schickard, Freiburg, Germany, Freiburg Center for Interactive Materials and Bioinspired Technologies (FIT), Freiburg, Germany

  ⨯
• Stefan Zimmermann

  Roles Conceptualization, Formal analysis, Funding acquisition, Project administration, Resources, Supervision, Validation, Writing – review & editing

  Affiliation Laboratory for MEMS Applications, IMTEK - Department of Microsystems Engineering, University of Freiburg, Freiburg, Germany

  ⨯