In vivo two-photon microscopic observation and ablation in deeper brain regions realized by modifications of excitation beam diameter and immersion liquid

• Kazushi Yamaguchi ,

  Contributed equally to this work with: Kazushi Yamaguchi, Ryoji Kitamura

  Roles Formal analysis, Investigation, Methodology, Resources, Software, Validation, Visualization, Writing – original draft, Writing – review & editing

  Affiliations Graduate School of Information Science and Technology, Hokkaido University, Sapporo, Hokkaido, Japan, Research Institute for Electronic Science, Hokkaido University, Sapporo, Hokkaido, Japan, Division of Biophotonics, National Institute for Physiological Sciences, National Institutes of Natural Sciences, Okazaki, Aichi, Japan

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• Ryoji Kitamura ,

  Contributed equally to this work with: Kazushi Yamaguchi, Ryoji Kitamura

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Software, Validation, Visualization, Writing – review & editing

  Affiliations Graduate School of Information Science and Technology, Hokkaido University, Sapporo, Hokkaido, Japan, Research Institute for Electronic Science, Hokkaido University, Sapporo, Hokkaido, Japan

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• Ryosuke Kawakami,

  Roles Conceptualization, Data curation, Funding acquisition, Methodology, Project administration, Resources, Visualization, Writing – original draft, Writing – review & editing

  Affiliations Graduate School of Information Science and Technology, Hokkaido University, Sapporo, Hokkaido, Japan, Research Institute for Electronic Science, Hokkaido University, Sapporo, Hokkaido, Japan, Graduate School of Medicine, Ehime University, Toon, Ehime, Japan

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• Kohei Otomo,

  Roles Conceptualization, Data curation, Project administration, Resources, Visualization, Writing – original draft, Writing – review & editing

  Affiliations Graduate School of Information Science and Technology, Hokkaido University, Sapporo, Hokkaido, Japan, Research Institute for Electronic Science, Hokkaido University, Sapporo, Hokkaido, Japan, Division of Biophotonics, National Institute for Physiological Sciences, National Institutes of Natural Sciences, Okazaki, Aichi, Japan, Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, Okazaki, Aichi, Japan, Department of Physiological Sciences, The Graduate School for Advanced Study, Hayama, Japan

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• Tomomi Nemoto

  Roles Conceptualization, Data curation, Funding acquisition, Methodology, Project administration, Resources, Supervision, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: tn@nips.ac.jp

  Affiliations Graduate School of Information Science and Technology, Hokkaido University, Sapporo, Hokkaido, Japan, Research Institute for Electronic Science, Hokkaido University, Sapporo, Hokkaido, Japan, Division of Biophotonics, National Institute for Physiological Sciences, National Institutes of Natural Sciences, Okazaki, Aichi, Japan, Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, Okazaki, Aichi, Japan, Department of Physiological Sciences, The Graduate School for Advanced Study, Hayama, Japan

  http://orcid.org/0000-0001-6102-1495

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