Preparation of a phosphotyrosine-protein standard for use in semiquantitative western blotting with enhanced chemiluminescence

Nancy Kendrick; Ginny Powers; Jon Johansen; Matt Hoelter; Andrew Koll; Sofia Carlson; Devika Channaveerappa; Costel C. Darie

doi:10.1371/journal.pone.0234645

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Open Access

Peer-reviewed

Research Article

Preparation of a phosphotyrosine-protein standard for use in semiquantitative western blotting with enhanced chemiluminescence

Nancy Kendrick ,

Roles Conceptualization, Formal analysis, Funding acquisition, Investigation, Project administration, Resources, Supervision, Visualization, Writing – original draft, Writing – review & editing

* E-mail: nancy@kendricklabs.com (NK); cdarie@clarkson.edu (CCD)

Affiliation Kendrick Labs, Inc, Madison, WI, United States of America
⨯
Ginny Powers,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliation Kendrick Labs, Inc, Madison, WI, United States of America
⨯
Jon Johansen,

Roles Conceptualization, Investigation, Methodology, Project administration, Supervision, Writing – review & editing

Affiliation Kendrick Labs, Inc, Madison, WI, United States of America
⨯
Matt Hoelter,

Roles Conceptualization, Investigation, Methodology, Project administration, Supervision, Writing – review & editing

Affiliation Kendrick Labs, Inc, Madison, WI, United States of America
⨯
Andrew Koll,

Roles Data curation, Formal analysis, Investigation, Methodology, Project administration, Validation, Writing – review & editing

Affiliation Kendrick Labs, Inc, Madison, WI, United States of America
⨯
Sofia Carlson,

Roles Data curation, Formal analysis, Investigation, Validation, Visualization, Writing – review & editing

Affiliation Kendrick Labs, Inc, Madison, WI, United States of America
⨯
Devika Channaveerappa,

Roles Data curation, Formal analysis, Visualization, Writing – review & editing

Affiliation Biochemistry & Proteomics Group, Department of Chemistry & Biomolecular Science, Clarkson University, Potsdam, NY, United States of America
⨯
Costel C. Darie

Roles Conceptualization, Formal analysis, Funding acquisition, Methodology, Project administration, Resources, Supervision, Visualization, Writing – original draft, Writing – review & editing

* E-mail: nancy@kendricklabs.com (NK); cdarie@clarkson.edu (CCD)

Affiliation Biochemistry & Proteomics Group, Department of Chemistry & Biomolecular Science, Clarkson University, Potsdam, NY, United States of America

http://orcid.org/0000-0001-6402-2311

⨯

Preparation of a phosphotyrosine-protein standard for use in semiquantitative western blotting with enhanced chemiluminescence

Nancy Kendrick,
Ginny Powers,
Jon Johansen,
Matt Hoelter,
Andrew Koll,
Sofia Carlson,
Devika Channaveerappa,
Costel C. Darie

Published: June 18, 2020
https://doi.org/10.1371/journal.pone.0234645

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Phosphorylation
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Tyrosine
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Gel electrophoresis
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Lung and intrathoracic tumors
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SDS polyacrylamide gel electrophoresis
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Cysteine
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Cancers and neoplasms
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Membrane staining
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