Identification of a reliable fixative solution to preserve the complex architecture of bacterial biofilms for scanning electron microscopy evaluation

Rohana P. Dassanayake; Shollie M. Falkenberg; Judith A. Stasko; Adrienne L. Shircliff; John D. Lippolis; Robert E. Briggs

doi:10.1371/journal.pone.0233973

Loading metrics

Open Access

Peer-reviewed

Research Article

Identification of a reliable fixative solution to preserve the complex architecture of bacterial biofilms for scanning electron microscopy evaluation

Rohana P. Dassanayake ,

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

* E-mail: rohana.dassanayake@usda.gov

Affiliation Ruminant Diseases and Immunology Research Unit, National Animal Disease Center, United States Department of Agriculture, Agricultural Research Service, Ames, Iowa, United States of America

http://orcid.org/0000-0002-8250-4370

⨯
Shollie M. Falkenberg,

Roles Formal analysis, Investigation, Resources, Writing – review & editing

Affiliation Ruminant Diseases and Immunology Research Unit, National Animal Disease Center, United States Department of Agriculture, Agricultural Research Service, Ames, Iowa, United States of America
⨯
Judith A. Stasko,

Roles Formal analysis, Investigation, Methodology, Resources, Validation, Visualization, Writing – review & editing

Affiliation Microscopy Services Laboratory, National Animal Disease Center, United States Department of Agriculture, Agricultural Research Service, Ames, Iowa, United States of America
⨯
Adrienne L. Shircliff,

Roles Formal analysis, Investigation, Resources, Validation, Visualization, Writing – review & editing

Affiliation Microscopy Services Laboratory, National Animal Disease Center, United States Department of Agriculture, Agricultural Research Service, Ames, Iowa, United States of America
⨯
John D. Lippolis,

Roles Funding acquisition, Investigation, Resources, Writing – review & editing

Affiliation Ruminant Diseases and Immunology Research Unit, National Animal Disease Center, United States Department of Agriculture, Agricultural Research Service, Ames, Iowa, United States of America
⨯
Robert E. Briggs

Roles Funding acquisition, Investigation, Resources, Writing – review & editing

Affiliation Ruminant Diseases and Immunology Research Unit, National Animal Disease Center, United States Department of Agriculture, Agricultural Research Service, Ames, Iowa, United States of America
⨯

Identification of a reliable fixative solution to preserve the complex architecture of bacterial biofilms for scanning electron microscopy evaluation

Rohana P. Dassanayake,
Shollie M. Falkenberg,
Judith A. Stasko,
Adrienne L. Shircliff,
John D. Lippolis,
Robert E. Briggs

Published: May 29, 2020
https://doi.org/10.1371/journal.pone.0233973

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Bacterial biofilms
Is the Subject Area "Bacterial biofilms" applicable to this article?

Thanks for your feedback.
Staphylococcus aureus
Is the Subject Area "Staphylococcus aureus" applicable to this article?

Thanks for your feedback.
Exopolysaccharides
Is the Subject Area "Exopolysaccharides" applicable to this article?

Thanks for your feedback.
Scanning electron microscopy
Is the Subject Area "Scanning electron microscopy" applicable to this article?

Thanks for your feedback.
Confocal laser microscopy
Is the Subject Area "Confocal laser microscopy" applicable to this article?

Thanks for your feedback.
Laboratory glassware
Is the Subject Area "Laboratory glassware" applicable to this article?

Thanks for your feedback.
Bovine mastitis
Is the Subject Area "Bovine mastitis" applicable to this article?

Thanks for your feedback.
Biofilm culture
Is the Subject Area "Biofilm culture" applicable to this article?

Thanks for your feedback.