Gene editing in CHO cells to prevent proteolysis and enhance glycosylation: Production of HIV envelope proteins as vaccine immunogens

Sophia W. Li; Meredith Wright; John F. Healey; Jennie M. Hutchinson; Sara O’Rourke; Kathryn A. Mesa; Pete Lollar; Phillip W. Berman

doi:10.1371/journal.pone.0233866

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Open Access

Peer-reviewed

Research Article

Gene editing in CHO cells to prevent proteolysis and enhance glycosylation: Production of HIV envelope proteins as vaccine immunogens

Sophia W. Li ,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Visualization, Writing – original draft, Writing – review & editing

* E-mail: sli140@ucsc.edu

Affiliations Department of Chemistry and Biochemistry, University of California Santa Cruz, Santa Cruz, California, United States of America, Department of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, California, United States of America

http://orcid.org/0000-0002-5873-1602

⨯
Meredith Wright,

Roles Investigation, Methodology

Affiliation Department of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, California, United States of America
⨯
John F. Healey,

Roles Formal analysis, Investigation, Methodology, Resources, Validation

Affiliation Department of Pediatrics, Emory University, Atlanta, Georgia, United States of America
⨯
Jennie M. Hutchinson,

Roles Formal analysis, Investigation, Methodology

Affiliation Department of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, California, United States of America
⨯
Sara O’Rourke,

Roles Investigation, Methodology

Affiliation Department of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, California, United States of America
⨯
Kathryn A. Mesa,

Roles Investigation

Affiliation Department of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, California, United States of America
⨯
Pete Lollar,

Roles Formal analysis, Investigation, Methodology, Supervision, Visualization

Affiliation Department of Pediatrics, Emory University, Atlanta, Georgia, United States of America
⨯
Phillip W. Berman

Roles Conceptualization, Supervision, Writing – review & editing

Affiliation Department of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, California, United States of America
⨯

Gene editing in CHO cells to prevent proteolysis and enhance glycosylation: Production of HIV envelope proteins as vaccine immunogens

Sophia W. Li,
Meredith Wright,
John F. Healey,
Jennie M. Hutchinson,
Sara O’Rourke,
Kathryn A. Mesa,
Pete Lollar,
Phillip W. Berman

Published: May 29, 2020
https://doi.org/10.1371/journal.pone.0233866

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CHO cells
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Proteolysis
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Thrombin
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Proteases
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Recombinant proteins
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Cloning
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HIV
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Glycosylation
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