Automated image analysis of stained cytospins to quantify Schwann cell purity and proliferation

Tamara Weiss; Lorenz Semmler; Flavia Millesi; Anda Mann; Maximilian Haertinger; Manuel Salzmann; Christine Radtke

doi:10.1371/journal.pone.0233647

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Open Access

Peer-reviewed

Research Article

Automated image analysis of stained cytospins to quantify Schwann cell purity and proliferation

Tamara Weiss ,

Contributed equally to this work with: Tamara Weiss, Lorenz Semmler

Roles Conceptualization, Formal analysis, Methodology, Project administration, Validation, Visualization, Writing – original draft

* E-mail: tamara.weiss@meduniwien.ac.at

Affiliation Research Laboratory of the Division of Plastic and Reconstructive Surgery, Department of Surgery, Medical University of Vienna, Vienna, Austria

http://orcid.org/0000-0001-7046-3412

⨯
Lorenz Semmler ,

Contributed equally to this work with: Tamara Weiss, Lorenz Semmler

Roles Data curation, Investigation, Methodology, Software, Writing – review & editing

Affiliation Research Laboratory of the Division of Plastic and Reconstructive Surgery, Department of Surgery, Medical University of Vienna, Vienna, Austria
⨯
Flavia Millesi,

Roles Investigation, Writing – review & editing

Affiliation Research Laboratory of the Division of Plastic and Reconstructive Surgery, Department of Surgery, Medical University of Vienna, Vienna, Austria
⨯
Anda Mann,

Roles Investigation

Affiliation Research Laboratory of the Division of Plastic and Reconstructive Surgery, Department of Surgery, Medical University of Vienna, Vienna, Austria
⨯
Maximilian Haertinger,

Roles Investigation

Affiliation Research Laboratory of the Division of Plastic and Reconstructive Surgery, Department of Surgery, Medical University of Vienna, Vienna, Austria

http://orcid.org/0000-0003-2478-6477

⨯
Manuel Salzmann,

Roles Methodology, Software, Writing – review & editing

Affiliation Institute of Vascular Biology and Thrombosis Research, Medical University of Vienna, Vienna, Austria
⨯
Christine Radtke

Roles Funding acquisition, Supervision, Writing – review & editing

Affiliations Research Laboratory of the Division of Plastic and Reconstructive Surgery, Department of Surgery, Medical University of Vienna, Vienna, Austria, Division of Plastic and Reconstructive Surgery, Department of Surgery, Medical University of Vienna, Vienna, Austria
⨯

Automated image analysis of stained cytospins to quantify Schwann cell purity and proliferation

Tamara Weiss,
Lorenz Semmler,
Flavia Millesi,
Anda Mann,
Maximilian Haertinger,
Manuel Salzmann,
Christine Radtke

Published: May 22, 2020
https://doi.org/10.1371/journal.pone.0233647

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Cell staining
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Nuclear staining
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DAPI staining
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Immunofluorescence staining
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