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Open Access
Peer-reviewed
Research Article
A qPCR assay for Bordetella pertussis cells that enumerates both live and dead bacteria
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Stacy Ramkissoon,
Roles Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing
Affiliations Milner Centre for Evolution, University of Bath, Bath, United Kingdom, Department of Biology and Biochemistry, University of Bath, Bath, United Kingdom
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Iain MacArthur,
Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Supervision, Validation, Visualization
Affiliations Milner Centre for Evolution, University of Bath, Bath, United Kingdom, Department of Biology and Biochemistry, University of Bath, Bath, United Kingdom
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Muktar Ibrahim,
Roles Formal analysis, Investigation, Resources
Affiliations Faculty of Medicine and Institute for Life Sciences, University of Southampton, Southampton, United Kingdom, School of Clinical Experimental Sciences, University Hospital Southampton NHS Foundation Trust, Southampton General Hospital, University of Southampton, Southampton, United Kingdom, NIHR Clinical Research Facility, University Hospital Southampton NHS Foundation Trust, Southampton General Hospital, University of Southampton, Southampton, United Kingdom, NIHR Southampton Biomedical Research Centre, University Hospital Southampton NHS Foundation Trust, Southampton General Hospital, University of Southampton, Southampton, United Kingdom
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Hans de Graaf,
Roles Conceptualization, Investigation, Methodology, Project administration, Resources
Affiliations Faculty of Medicine and Institute for Life Sciences, University of Southampton, Southampton, United Kingdom, School of Clinical Experimental Sciences, University Hospital Southampton NHS Foundation Trust, Southampton General Hospital, University of Southampton, Southampton, United Kingdom, NIHR Clinical Research Facility, University Hospital Southampton NHS Foundation Trust, Southampton General Hospital, University of Southampton, Southampton, United Kingdom, NIHR Southampton Biomedical Research Centre, University Hospital Southampton NHS Foundation Trust, Southampton General Hospital, University of Southampton, Southampton, United Kingdom
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Robert C. Read,
Roles Conceptualization, Investigation, Methodology, Project administration, Resources
Affiliations Faculty of Medicine and Institute for Life Sciences, University of Southampton, Southampton, United Kingdom, School of Clinical Experimental Sciences, University Hospital Southampton NHS Foundation Trust, Southampton General Hospital, University of Southampton, Southampton, United Kingdom, NIHR Clinical Research Facility, University Hospital Southampton NHS Foundation Trust, Southampton General Hospital, University of Southampton, Southampton, United Kingdom, NIHR Southampton Biomedical Research Centre, University Hospital Southampton NHS Foundation Trust, Southampton General Hospital, University of Southampton, Southampton, United Kingdom
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Andrew Preston
Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Software, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing
* E-mail: a.preston@bath.ac.uk
Affiliations Milner Centre for Evolution, University of Bath, Bath, United Kingdom, Department of Biology and Biochemistry, University of Bath, Bath, United Kingdom
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A qPCR assay for Bordetella pertussis cells that enumerates both live and dead bacteria
- Stacy Ramkissoon,
- Iain MacArthur,
- Muktar Ibrahim,
- Hans de Graaf,
- Robert C. Read,
- Andrew Preston
- Published: April 30, 2020
- https://doi.org/10.1371/journal.pone.0232334
- See the preprint