Rapid and robust antibody Fab fragment crystallization utilizing edge-to-edge beta-sheet packing

Ricky Lieu; Stephen Antonysamy; Zhanna Druzina; Carolyn Ho; N. Rebecca Kang; Anna Pustilnik; Jing Wang; Shane Atwell

doi:10.1371/journal.pone.0232311

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Open Access

Peer-reviewed

Research Article

Rapid and robust antibody Fab fragment crystallization utilizing edge-to-edge beta-sheet packing

Ricky Lieu,

Roles Conceptualization, Investigation, Writing – original draft, Writing – review & editing

Affiliation Biotechnology Discovery Research, Applied Molecular Evolution, Eli Lilly and Company, San Diego, CA, United States of America
⨯
Stephen Antonysamy,

Roles Investigation

Affiliation Discovery Chemistry Research and Technologies, Eli Lilly and Company Corporate Center, Indianapolis, IN, United States of America
⨯
Zhanna Druzina,

Roles Investigation, Writing – original draft

Current address: Protein Engineering, Aro Biotherapeutics Company, Philadelphia, PA, United States of America

Affiliation Discovery Chemistry Research and Technologies, Eli Lilly and Company Corporate Center, Indianapolis, IN, United States of America
⨯
Carolyn Ho,

Roles Investigation, Writing – original draft

Affiliation Biotechnology Discovery Research, Applied Molecular Evolution, Eli Lilly and Company, San Diego, CA, United States of America
⨯
N. Rebecca Kang,

Roles Investigation

Current address: Department of Biomedical Engineering, Boston University, Boston, MA, United States of America

Affiliation Biotechnology Discovery Research, Applied Molecular Evolution, Eli Lilly and Company, San Diego, CA, United States of America
⨯
Anna Pustilnik,

Roles Investigation, Writing – original draft

Affiliation Discovery Chemistry Research and Technologies, Eli Lilly and Company Corporate Center, Indianapolis, IN, United States of America
⨯
Jing Wang,

Roles Data curation, Investigation

Affiliation Discovery Chemistry Research and Technologies, Eli Lilly and Company Corporate Center, Indianapolis, IN, United States of America
⨯
Shane Atwell

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Visualization, Writing – original draft, Writing – review & editing

* E-mail: satwell@lilly.com

Affiliation Biotechnology Discovery Research, Applied Molecular Evolution, Eli Lilly and Company, San Diego, CA, United States of America

http://orcid.org/0000-0002-2578-2462

⨯

Rapid and robust antibody Fab fragment crystallization utilizing edge-to-edge beta-sheet packing

Ricky Lieu,
Stephen Antonysamy,
Zhanna Druzina,
Carolyn Ho,
N. Rebecca Kang,
Anna Pustilnik,
Jing Wang,
Shane Atwell

Published: September 11, 2020
https://doi.org/10.1371/journal.pone.0232311

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