Challenges associated with homologous directed repair using CRISPR-Cas9 and TALEN to edit the DMD genetic mutation in canine Duchenne muscular dystrophy

Sara Mata López; Cynthia Balog-Alvarez; Stanislav Vitha; Amanda K. Bettis; Emily H. Canessa; Joe N. Kornegay; Peter P. Nghiem

doi:10.1371/journal.pone.0228072

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Open Access

Peer-reviewed

Research Article

Challenges associated with homologous directed repair using CRISPR-Cas9 and TALEN to edit the DMD genetic mutation in canine Duchenne muscular dystrophy

Sara Mata López,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Software, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliation Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, United States of America

http://orcid.org/0000-0002-0280-7217

⨯
Cynthia Balog-Alvarez,

Roles Methodology, Resources, Supervision

Affiliation Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, United States of America
⨯
Stanislav Vitha,

Roles Data curation, Software

Affiliation Microscopy and Imaging Center, Texas A&M University, College Station, TX, United States of America
⨯
Amanda K. Bettis,

Roles Methodology

Affiliation Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, United States of America
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Emily H. Canessa,

Roles Data curation, Methodology, Software

Affiliation Department of Pharmaceutical Sciences, School of Pharmacy and Pharmaceutical Sciences, Binghamton University, Johnson City, NY, United States of America
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Joe N. Kornegay,

Roles Project administration, Supervision, Writing – review & editing

Affiliation Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, United States of America
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Peter P. Nghiem

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Project administration, Software, Supervision, Validation, Writing – review & editing

* E-mail: pnghiem@tamu.edu

Affiliation Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, United States of America
⨯

Challenges associated with homologous directed repair using CRISPR-Cas9 and TALEN to edit the DMD genetic mutation in canine Duchenne muscular dystrophy

Sara Mata López,
Cynthia Balog-Alvarez,
Stanislav Vitha,
Amanda K. Bettis,
Emily H. Canessa,
Joe N. Kornegay,
Peter P. Nghiem

Published: January 21, 2020
https://doi.org/10.1371/journal.pone.0228072

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Dystrophin
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Dogs
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TALENs
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Muscle proteins
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Myoblasts
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Polymerase chain reaction
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