A versatile modular vector set for optimizing protein expression among bacterial, yeast, insect and mammalian hosts

Márk Somogyi; Tamás Szimler; Attila Baksa; Barbara M. Végh; Tamás Bakos; Katalin Paréj; Csaba Ádám; Áron Zsigmond; Márton Megyeri; Beáta Flachner; Ráchel Sajó; Éva Gráczer; Péter Závodszky; István Hajdú; László Beinrohr

doi:10.1371/journal.pone.0227110

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Open Access

Peer-reviewed

Research Article

A versatile modular vector set for optimizing protein expression among bacterial, yeast, insect and mammalian hosts

Márk Somogyi,

Roles Data curation, Formal analysis, Investigation, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliation Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Pázmány Péter sétány, Budapest, Hungary
⨯
Tamás Szimler,

Roles Formal analysis, Investigation, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliation Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Pázmány Péter sétány, Budapest, Hungary
⨯
Attila Baksa,

Roles Investigation, Validation

Affiliation Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Pázmány Péter sétány, Budapest, Hungary
⨯
Barbara M. Végh,

Roles Investigation, Validation

Affiliation Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Pázmány Péter sétány, Budapest, Hungary
⨯
Tamás Bakos,

Roles Investigation, Validation

Affiliation Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Pázmány Péter sétány, Budapest, Hungary
⨯
Katalin Paréj,

Roles Investigation, Validation

Affiliation Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Pázmány Péter sétány, Budapest, Hungary
⨯
Csaba Ádám,

Roles Investigation

Affiliation Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Pázmány Péter sétány, Budapest, Hungary
⨯
Áron Zsigmond,

Roles Investigation

Affiliation Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Pázmány Péter sétány, Budapest, Hungary
⨯
Márton Megyeri,

Roles Investigation

Affiliation Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Pázmány Péter sétány, Budapest, Hungary
⨯
Beáta Flachner,

Roles Investigation

Affiliation Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Pázmány Péter sétány, Budapest, Hungary
⨯
Ráchel Sajó,

Roles Investigation

Affiliation Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Pázmány Péter sétány, Budapest, Hungary
⨯
Éva Gráczer,

Roles Investigation

Affiliation Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Pázmány Péter sétány, Budapest, Hungary

http://orcid.org/0000-0002-8312-5591

⨯
Péter Závodszky,

Roles Conceptualization, Supervision, Writing – review & editing

Affiliation Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Pázmány Péter sétány, Budapest, Hungary
⨯
István Hajdú ,

Roles Supervision, Writing – review & editing

* E-mail: laszlo@beinrohr.com (LB); hajdu.istvan@ttk.mta.hu(IH)

Affiliation Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Pázmány Péter sétány, Budapest, Hungary

http://orcid.org/0000-0003-2312-0833

⨯
László Beinrohr

Roles Conceptualization, Investigation, Methodology, Project administration, Supervision, Writing – original draft, Writing – review & editing

* E-mail: laszlo@beinrohr.com (LB); hajdu.istvan@ttk.mta.hu(IH)

Affiliation Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Pázmány Péter sétány, Budapest, Hungary
⨯

A versatile modular vector set for optimizing protein expression among bacterial, yeast, insect and mammalian hosts

Márk Somogyi,
Tamás Szimler,
Attila Baksa,
Barbara M. Végh,
Tamás Bakos,
Katalin Paréj,
Csaba Ádám,
Áron Zsigmond,
Márton Megyeri,
Beáta Flachner

Published: December 30, 2019
https://doi.org/10.1371/journal.pone.0227110

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Insect vectors
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Protein expression
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Pichia pastoris
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