A homogeneous SIRPα-CD47 cell-based, ligand-binding assay: Utility for small molecule drug development in immuno-oncology

Teresa L. Burgess; Joshua D. Amason; Jeffrey S. Rubin; Damien Y. Duveau; Laurence Lamy; David D. Roberts; Catherine L. Farrell; James Inglese; Craig J. Thomas; Thomas W. Miller

doi:10.1371/journal.pone.0226661

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Open Access

Peer-reviewed

Research Article

A homogeneous SIRPα-CD47 cell-based, ligand-binding assay: Utility for small molecule drug development in immuno-oncology

Teresa L. Burgess,

Roles Conceptualization, Formal analysis, Funding acquisition, Project administration, Supervision, Writing – original draft, Writing – review & editing

Affiliation Paradigm Shift Therapeutics LLC, Rockville, Maryland, United States of America
⨯
Joshua D. Amason,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing – original draft

Current address: Department of Ophthalmology, Duke University School of Medicine, Durham, North Carolina, United States of America.

Affiliations Paradigm Shift Therapeutics LLC, Rockville, Maryland, United States of America, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America
⨯
Jeffrey S. Rubin,

Roles Conceptualization, Formal analysis, Funding acquisition, Methodology, Validation, Writing – original draft, Writing – review & editing

Affiliation Paradigm Shift Therapeutics LLC, Rockville, Maryland, United States of America
⨯
Damien Y. Duveau,

Roles Data curation, Formal analysis, Investigation, Methodology, Writing – original draft, Writing – review & editing

Affiliation Division of Preclinical Innovation, National Center for Advancing Translational Studies, National Institutes of Health, Rockville, Maryland, United States of America
⨯
Laurence Lamy,

Roles Data curation, Formal analysis, Investigation, Methodology, Writing – review & editing

Affiliation Division of Preclinical Innovation, National Center for Advancing Translational Studies, National Institutes of Health, Rockville, Maryland, United States of America
⨯
David D. Roberts,

Roles Conceptualization, Funding acquisition, Methodology, Resources, Writing – review & editing

Affiliation Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America
⨯
Catherine L. Farrell,

Roles Conceptualization, Funding acquisition, Methodology, Project administration, Resources, Writing – review & editing

Affiliation Paradigm Shift Therapeutics LLC, Rockville, Maryland, United States of America
⨯
James Inglese,

Roles Conceptualization, Methodology, Resources, Visualization, Writing – review & editing

Affiliation Division of Preclinical Innovation, National Center for Advancing Translational Studies, National Institutes of Health, Rockville, Maryland, United States of America

http://orcid.org/0000-0002-7332-5717

⨯
Craig J. Thomas,

Roles Resources, Supervision, Writing – review & editing

Affiliation Division of Preclinical Innovation, National Center for Advancing Translational Studies, National Institutes of Health, Rockville, Maryland, United States of America
⨯
Thomas W. Miller

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Software, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

* E-mail: thomas.miller@pstherx.com

Current address: Institut Paoli-Calmettes, Cancer Research Center Marseille, Marseille, France

Affiliations Paradigm Shift Therapeutics LLC, Rockville, Maryland, United States of America, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America

http://orcid.org/0000-0001-8645-2785

⨯

A homogeneous SIRPα-CD47 cell-based, ligand-binding assay: Utility for small molecule drug development in immuno-oncology

Teresa L. Burgess,
Joshua D. Amason,
Jeffrey S. Rubin,
Damien Y. Duveau,
Laurence Lamy,
David D. Roberts,
Catherine L. Farrell,
James Inglese,
Craig J. Thomas,
Thomas W. Miller

Published: April 2, 2020
https://doi.org/10.1371/journal.pone.0226661

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