Binding and functional profiling of antibody mutants guides selection of optimal candidates as antibody drug conjugates

John C. Zwaagstra; Traian Sulea; Jason Baardsnes; Stevo Radinovic; Yuneivy Cepero-Donates; Alma Robert; Maureen D. O’Connor-McCourt; Ilia A. Tikhomirov; Maria Luz. Jaramillo

doi:10.1371/journal.pone.0226593

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Open Access

Peer-reviewed

Research Article

Binding and functional profiling of antibody mutants guides selection of optimal candidates as antibody drug conjugates

John C. Zwaagstra ,

Roles Conceptualization, Data curation, Project administration, Supervision, Validation, Writing – original draft, Writing – review & editing

* E-mail: john.zwaagstra@nrc-cnrc.gc.ca (JZ); maria.jaramillo@nrc-cnrc.gc.ca (MJ)

Affiliation Human Health Therapeutics Research Centre, National Research Council Canada, Montreal, Quebec, Canada

http://orcid.org/0000-0002-6551-063X

⨯
Traian Sulea,

Roles Conceptualization, Data curation, Writing – review & editing

Affiliation Human Health Therapeutics Research Centre, National Research Council Canada, Montreal, Quebec, Canada
⨯
Jason Baardsnes,

Roles Data curation, Formal analysis, Methodology, Writing – review & editing

Affiliation Human Health Therapeutics Research Centre, National Research Council Canada, Montreal, Quebec, Canada
⨯
Stevo Radinovic,

Roles Data curation, Formal analysis, Methodology, Writing – review & editing

Affiliation Human Health Therapeutics Research Centre, National Research Council Canada, Montreal, Quebec, Canada
⨯
Yuneivy Cepero-Donates,

Roles Data curation, Formal analysis, Methodology

Affiliation Human Health Therapeutics Research Centre, National Research Council Canada, Montreal, Quebec, Canada
⨯
Alma Robert,

Roles Data curation, Formal analysis, Methodology, Writing – review & editing

Affiliation Human Health Therapeutics Research Centre, National Research Council Canada, Montreal, Quebec, Canada
⨯
Maureen D. O’Connor-McCourt,

Roles Conceptualization, Formal analysis, Writing – review & editing

Affiliation Forbius, Montreal, Quebec, Canada
⨯
Ilia A. Tikhomirov,

Roles Conceptualization, Formal analysis, Writing – review & editing

Affiliation Forbius, Montreal, Quebec, Canada
⨯
Maria Luz. Jaramillo

Roles Conceptualization, Data curation, Formal analysis, Writing – original draft, Writing – review & editing

* E-mail: john.zwaagstra@nrc-cnrc.gc.ca (JZ); maria.jaramillo@nrc-cnrc.gc.ca (MJ)

Affiliation Human Health Therapeutics Research Centre, National Research Council Canada, Montreal, Quebec, Canada
⨯

Binding and functional profiling of antibody mutants guides selection of optimal candidates as antibody drug conjugates

John C. Zwaagstra,
Traian Sulea,
Jason Baardsnes,
Stevo Radinovic,
Yuneivy Cepero-Donates,
Alma Robert,
Maureen D. O’Connor-McCourt,
Ilia A. Tikhomirov,
Maria Luz. Jaramillo

Published: December 31, 2019
https://doi.org/10.1371/journal.pone.0226593

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Cell binding
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Flow cytometry
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Toxicity
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Cell binding assay
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Antibody therapy
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