Optimizing sgRNA length to improve target specificity and efficiency for the GGTA1 gene using the CRISPR/Cas9 gene editing system

• Anders W. Matson,

  Roles Data curation, Formal analysis, Investigation, Methodology, Validation, Writing – original draft, Writing – review & editing

  Affiliation Schulze Diabetes Institute, Department of Surgery, University of Minnesota School of Medicine, Minneapolis, MN, United States of America

  http://orcid.org/0000-0001-8470-5822

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• Nora Hosny,

  Roles Data curation, Formal analysis, Investigation, Writing – review & editing

  Affiliations Schulze Diabetes Institute, Department of Surgery, University of Minnesota School of Medicine, Minneapolis, MN, United States of America, Department of Medical Biochemistry and Molecular Biology, Suez Canal University, Faculty of Medicine, Ismailia, Egypt

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• Zachary A. Swanson,

  Roles Methodology, Validation

  Affiliation Schulze Diabetes Institute, Department of Surgery, University of Minnesota School of Medicine, Minneapolis, MN, United States of America

  http://orcid.org/0000-0002-2580-2865

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• Bernhard J. Hering,

  Roles Writing – review & editing

  Affiliation Schulze Diabetes Institute, Department of Surgery, University of Minnesota School of Medicine, Minneapolis, MN, United States of America

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• Christopher Burlak

  Roles Conceptualization, Funding acquisition, Supervision, Writing – original draft, Writing – review & editing

  * E-mail: cburlak@umn.edu

  Affiliation Schulze Diabetes Institute, Department of Surgery, University of Minnesota School of Medicine, Minneapolis, MN, United States of America

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