Generation of deletions and precise point mutations in Dictyostelium discoideum using the CRISPR nickase

• Hoshie Iriki,

  Roles Conceptualization, Formal analysis, Investigation, Validation, Visualization, Writing – original draft, Writing – review & editing

  Affiliation Department of Biology, Faculty of Science, Toho University, Funabashi, Chiba, Japan

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• Takefumi Kawata,

  Roles Resources, Writing – review & editing

  Affiliation Department of Biology, Faculty of Science, Toho University, Funabashi, Chiba, Japan

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• Tetsuya Muramoto

  Roles Conceptualization, Investigation, Resources, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: tetsuya.muramoto@sci.toho-u.ac.jp

  Affiliation Department of Biology, Faculty of Science, Toho University, Funabashi, Chiba, Japan

  http://orcid.org/0000-0001-9624-8577

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