CRISPR/Cas9-based editing of a sensitive transcriptional regulatory element to achieve cell type-specific knockdown of the NEMO scaffold protein

• Milad Babaei ,

  Contributed equally to this work with: Milad Babaei, Yuekun Liu

  Roles Conceptualization, Formal analysis, Investigation, Writing – review & editing

  Affiliation Department of Biology, Boston University, Boston, Massachusetts, United States of America

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• Yuekun Liu ,

  Contributed equally to this work with: Milad Babaei, Yuekun Liu

  Roles Conceptualization, Formal analysis, Investigation, Methodology, Writing – review & editing

  Affiliation Department of Biology, Boston University, Boston, Massachusetts, United States of America

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• Shelly M. Wuerzberger-Davis,

  Roles Conceptualization, Formal analysis, Investigation, Methodology, Writing – review & editing

  Affiliation Department of Oncology, McArdle Laboratory for Cancer Research, University of Wisconsin Carbone Cancer Center, University of Wisconsin, Madison, Wisconsin, United States of America

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• Ethan Z. McCaslin,

  Roles Investigation, Methodology

  Affiliation Department of Biology, Boston University, Boston, Massachusetts, United States of America

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• Christopher J. DiRusso,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing – review & editing

  Affiliation Department of Biology, Boston University, Boston, Massachusetts, United States of America

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• Alan T. Yeo,

  Roles Conceptualization, Methodology

  Affiliation Department of Biology, Boston University, Boston, Massachusetts, United States of America

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• Larisa Kagermazova,

  Roles Conceptualization, Methodology

  Affiliation Department of Biology, Boston University, Boston, Massachusetts, United States of America

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• Shigeki Miyamoto,

  Roles Conceptualization, Funding acquisition, Writing – review & editing

  Affiliation Department of Oncology, McArdle Laboratory for Cancer Research, University of Wisconsin Carbone Cancer Center, University of Wisconsin, Madison, Wisconsin, United States of America

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• Thomas D. Gilmore

  Roles Conceptualization, Funding acquisition, Investigation, Methodology, Resources, Supervision, Writing – original draft, Writing – review & editing

  * E-mail: gilmore@bu.edu

  Affiliation Department of Biology, Boston University, Boston, Massachusetts, United States of America

  http://orcid.org/0000-0002-1910-768X

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