Evaluation of KRAS, NRAS and BRAF hotspot mutations detection for patients with metastatic colorectal cancer using direct DNA pipetting in a fully-automated platform and Next-Generation Sequencing for laboratory workflow optimisation

Pauline Gilson; Claire Franczak; Ludovic Dubouis; Marie Husson; Marie Rouyer; Jessica Demange; Marie Perceau; Agnès Leroux; Jean-Louis Merlin; Alexandre Harlé

doi:10.1371/journal.pone.0219204

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Open Access

Peer-reviewed

Research Article

Evaluation of KRAS, NRAS and BRAF hotspot mutations detection for patients with metastatic colorectal cancer using direct DNA pipetting in a fully-automated platform and Next-Generation Sequencing for laboratory workflow optimisation

Pauline Gilson ,

Contributed equally to this work with: Pauline Gilson, Claire Franczak

Roles Writing – original draft

Affiliations Université de Lorraine, CNRS UMR 7039 CRAN, Institut de Cancérologie de Lorraine, Service de Biopathologie, Vandœuvre-lès-Nancy, France, Institut de Cancérologie de Lorraine, Service de Biopathologie, Vandœuvre-lès-Nancy, France
⨯
Claire Franczak ,

Contributed equally to this work with: Pauline Gilson, Claire Franczak

Roles Writing – original draft

Affiliation Institut de Cancérologie de Lorraine, Service de Biopathologie, Vandœuvre-lès-Nancy, France
⨯
Ludovic Dubouis,

Roles Formal analysis, Methodology

Affiliation Institut de Cancérologie de Lorraine, Service de Biopathologie, Vandœuvre-lès-Nancy, France
⨯
Marie Husson,

Roles Conceptualization, Formal analysis

Affiliation Institut de Cancérologie de Lorraine, Service de Biopathologie, Vandœuvre-lès-Nancy, France
⨯
Marie Rouyer,

Roles Conceptualization, Methodology

Affiliation Institut de Cancérologie de Lorraine, Service de Biopathologie, Vandœuvre-lès-Nancy, France
⨯
Jessica Demange,

Roles Conceptualization, Methodology

Affiliation Institut de Cancérologie de Lorraine, Service de Biopathologie, Vandœuvre-lès-Nancy, France
⨯
Marie Perceau,

Roles Investigation

Affiliation Institut de Cancérologie de Lorraine, Service de Biopathologie, Vandœuvre-lès-Nancy, France
⨯
Agnès Leroux,

Roles Supervision, Validation, Writing – review & editing

Affiliation Institut de Cancérologie de Lorraine, Service de Biopathologie, Vandœuvre-lès-Nancy, France
⨯
Jean-Louis Merlin,

Roles Supervision, Validation, Writing – review & editing

Affiliations Université de Lorraine, CNRS UMR 7039 CRAN, Institut de Cancérologie de Lorraine, Service de Biopathologie, Vandœuvre-lès-Nancy, France, Institut de Cancérologie de Lorraine, Service de Biopathologie, Vandœuvre-lès-Nancy, France
⨯
Alexandre Harlé

Roles Supervision, Validation, Writing – review & editing

* E-mail: a.harle@nancy.unicancer.fr

Affiliations Université de Lorraine, CNRS UMR 7039 CRAN, Institut de Cancérologie de Lorraine, Service de Biopathologie, Vandœuvre-lès-Nancy, France, Institut de Cancérologie de Lorraine, Service de Biopathologie, Vandœuvre-lès-Nancy, France

http://orcid.org/0000-0001-9548-6522

⨯

Evaluation of KRAS, NRAS and BRAF hotspot mutations detection for patients with metastatic colorectal cancer using direct DNA pipetting in a fully-automated platform and Next-Generation Sequencing for laboratory workflow optimisation

Pauline Gilson,
Claire Franczak,
Ludovic Dubouis,
Marie Husson,
Marie Rouyer,
Jessica Demange,
Marie Perceau,
Agnès Leroux,
Jean-Louis Merlin,
Alexandre Harlé

Published: July 2, 2019
https://doi.org/10.1371/journal.pone.0219204

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Mutation detection
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Mutation
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DNA extraction
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Next-generation sequencing
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Colorectal cancer
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Cancers and neoplasms
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Rectal cancer
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Cancer treatment
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