Lessening of porcine epidemic diarrhoea virus susceptibility in piglets after editing of the CMP-N-glycolylneuraminic acid hydroxylase gene with CRISPR/Cas9 to nullify N-glycolylneuraminic acid expression

• Ching-Fu Tu ,

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: cftu@mail.atri.org.tw

  Affiliation Division of Animal Technology, Animal Technology Laboratories, Agricultural Technology Research Institute, Xiangshan Dist., Hsinchu, Taiwan, R.O.C

  http://orcid.org/0000-0001-5142-7264

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• Chin-kai Chuang ,

  Contributed equally to this work with: Chin-kai Chuang, Kai-Hsuan Hsiao, Chien-Hong Chen

  Roles Conceptualization, Investigation, Methodology, Validation

  Affiliation Division of Animal Technology, Animal Technology Laboratories, Agricultural Technology Research Institute, Xiangshan Dist., Hsinchu, Taiwan, R.O.C

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• Kai-Hsuan Hsiao ,

  Contributed equally to this work with: Chin-kai Chuang, Kai-Hsuan Hsiao, Chien-Hong Chen

  Roles Data curation, Formal analysis, Investigation, Methodology, Project administration, Writing – original draft

  Affiliations Division of Animal Technology, Animal Technology Laboratories, Agricultural Technology Research Institute, Xiangshan Dist., Hsinchu, Taiwan, R.O.C, Department of Life Sciences, National Chung Hsing University, South Dist., Taichung, Taiwan, R.O.C

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• Chien-Hong Chen ,

  Contributed equally to this work with: Chin-kai Chuang, Kai-Hsuan Hsiao, Chien-Hong Chen

  Roles Data curation, Investigation, Methodology, Writing – review & editing

  Current address: Reproductive Medicine Center, Lee Women’s Hospital, Taichung, Taiwan, R.O.C.

  Affiliation Division of Animal Technology, Animal Technology Laboratories, Agricultural Technology Research Institute, Xiangshan Dist., Hsinchu, Taiwan, R.O.C

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• Chi-Min Chen,

  Roles Data curation, Formal analysis, Investigation, Methodology, Resources, Supervision, Validation, Writing – original draft

  Current address: Chao Kun Biotech Ltd., Taipei, Taiwan, R.O.C.

  Affiliation Division of Animal Medicine, Animal Technology Laboratories, Agricultural Technology Research Institute, Xiangshan Dist., Hsinchu, Taiwan, R.O.C

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• Su-Hei Peng,

  Roles Data curation, Formal analysis, Investigation, Methodology, Writing – original draft

  Affiliation Division of Animal Technology, Animal Technology Laboratories, Agricultural Technology Research Institute, Xiangshan Dist., Hsinchu, Taiwan, R.O.C

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• Yu-Hsiu Su,

  Roles Data curation, Formal analysis, Investigation, Methodology

  Affiliation Division of Animal Technology, Animal Technology Laboratories, Agricultural Technology Research Institute, Xiangshan Dist., Hsinchu, Taiwan, R.O.C

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• Ming-Tang Chiou,

  Roles Data curation, Investigation, Methodology, Resources, Validation

  Affiliation Department of Veterinary Medicine, College of Veterinary Medicine, National of Science and Technology, Pingtung, Taiwan, ROC

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• Chon-Ho Yen,

  Roles Data curation, Formal analysis, Investigation, Methodology, Validation, Writing – review & editing

  Affiliation Division of Animal Technology, Animal Technology Laboratories, Agricultural Technology Research Institute, Xiangshan Dist., Hsinchu, Taiwan, R.O.C

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• Shao-Wen Hung,

  Roles Data curation, Formal analysis, Investigation, Methodology, Software, Writing – original draft

  Affiliation Division of Animal Industry, Animal Technology Laboratories, Agricultural Technology Research Institute, Xiangshan Dist., Hsinchu, Taiwan, R.O.C

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• Tien-Shuh Yang,

  Roles Conceptualization, Formal analysis, Investigation, Supervision, Validation, Writing – original draft, Writing – review & editing

  Affiliations Division of Animal Technology, Animal Technology Laboratories, Agricultural Technology Research Institute, Xiangshan Dist., Hsinchu, Taiwan, R.O.C, Department of Biotechnology and Animal Science, National Ilan University, Yilan, Yilan, Taiwan, R.O.C

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• Chuan-Mu Chen

  Roles Conceptualization, Supervision, Writing – original draft, Writing – review & editing

  Affiliations Department of Life Sciences, National Chung Hsing University, South Dist., Taichung, Taiwan, R.O.C, The iEGG and Animal Biotechnology Center, National Chung Hsinh University, Taichung, Taiwan, R.O.C

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