Multiplex PCR methods for detection of several viruses associated with canine respiratory and enteric diseases

Xiangqi Hao; Ruohan Liu; Yuwei He; Xiangyu Xiao; Weiqi Xiao; Qingxu Zheng; Xi Lin; Pan Tao; Pei Zhou; Shoujun Li

doi:10.1371/journal.pone.0213295

Loading metrics

Open Access

Peer-reviewed

Research Article

Multiplex PCR methods for detection of several viruses associated with canine respiratory and enteric diseases

Xiangqi Hao,

Roles Investigation, Methodology, Writing – original draft

Affiliations College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, People’s Republic of China, Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province, People’s Republic of China, Guangdong Provincial Pet Engineering Technology Research Center, Guangzhou, Guangdong Province, People’s Republic of China
⨯
Ruohan Liu,

Roles Investigation, Methodology

Affiliations College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, People’s Republic of China, Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province, People’s Republic of China, Guangdong Provincial Pet Engineering Technology Research Center, Guangzhou, Guangdong Province, People’s Republic of China
⨯
Yuwei He,

Roles Methodology

Affiliations College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, People’s Republic of China, Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province, People’s Republic of China, Guangdong Provincial Pet Engineering Technology Research Center, Guangzhou, Guangdong Province, People’s Republic of China
⨯
Xiangyu Xiao,

Roles Methodology

Affiliations College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, People’s Republic of China, Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province, People’s Republic of China, Guangdong Provincial Pet Engineering Technology Research Center, Guangzhou, Guangdong Province, People’s Republic of China
⨯
Weiqi Xiao,

Roles Methodology

Affiliations College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, People’s Republic of China, Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province, People’s Republic of China, Guangdong Provincial Pet Engineering Technology Research Center, Guangzhou, Guangdong Province, People’s Republic of China
⨯
Qingxu Zheng,

Roles Methodology

Affiliations College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, People’s Republic of China, Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province, People’s Republic of China, Guangdong Provincial Pet Engineering Technology Research Center, Guangzhou, Guangdong Province, People’s Republic of China
⨯
Xi Lin,

Roles Methodology

Affiliations College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, People’s Republic of China, Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province, People’s Republic of China, Guangdong Provincial Pet Engineering Technology Research Center, Guangzhou, Guangdong Province, People’s Republic of China
⨯
Pan Tao,

Roles Methodology

Affiliations College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, People’s Republic of China, Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province, People’s Republic of China, Guangdong Provincial Pet Engineering Technology Research Center, Guangzhou, Guangdong Province, People’s Republic of China
⨯
Pei Zhou ,

Roles Conceptualization, Supervision, Writing – review & editing

* E-mail: zhoupei@scau.edu.cn (PZ); shoujunli@scau.edu.cn (SL)

Affiliations College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, People’s Republic of China, Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province, People’s Republic of China, Guangdong Provincial Pet Engineering Technology Research Center, Guangzhou, Guangdong Province, People’s Republic of China

http://orcid.org/0000-0002-9560-2826

⨯
Shoujun Li

Roles Conceptualization, Funding acquisition, Supervision, Writing – review & editing

* E-mail: zhoupei@scau.edu.cn (PZ); shoujunli@scau.edu.cn (SL)

Affiliations College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, People’s Republic of China, Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province, People’s Republic of China, Guangdong Provincial Pet Engineering Technology Research Center, Guangzhou, Guangdong Province, People’s Republic of China
⨯

Multiplex PCR methods for detection of several viruses associated with canine respiratory and enteric diseases

Xiangqi Hao,
Ruohan Liu,
Yuwei He,
Xiangyu Xiao,
Weiqi Xiao,
Qingxu Zheng,
Xi Lin,
Pan Tao,
Pei Zhou,
Shoujun Li

Published: March 4, 2019
https://doi.org/10.1371/journal.pone.0213295

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Respiratory infections
Is the Subject Area "Respiratory infections" applicable to this article?

Thanks for your feedback.
Dogs
Is the Subject Area "Dogs" applicable to this article?

Thanks for your feedback.
Polymerase chain reaction
Is the Subject Area "Polymerase chain reaction" applicable to this article?

Thanks for your feedback.
Viral pathogens
Is the Subject Area "Viral pathogens" applicable to this article?

Thanks for your feedback.
Co-infections
Is the Subject Area "Co-infections" applicable to this article?

Thanks for your feedback.
Pathogens
Is the Subject Area "Pathogens" applicable to this article?

Thanks for your feedback.
Canine distemper virus
Is the Subject Area "Canine distemper virus" applicable to this article?

Thanks for your feedback.
Canine coronavirus
Is the Subject Area "Canine coronavirus" applicable to this article?

Thanks for your feedback.