Urea-mediated dissociation alleviate the false-positive Treponema pallidum-specific antibodies detected by ELISA

Qiang Wang; Yan Lei; Xiaolan Lu; Guangrong Wang; Qin Du; Xiaolan Guo; Yan Xing; Guoyuan Zhang; Dongsheng Wang

doi:10.1371/journal.pone.0212893

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Open Access

Peer-reviewed

Research Article

Urea-mediated dissociation alleviate the false-positive Treponema pallidum-specific antibodies detected by ELISA

Qiang Wang ,

Contributed equally to this work with: Qiang Wang, Yan Lei

Roles Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Supervision, Writing – original draft, Writing – review & editing

Affiliations Department of Laboratory Medicine, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, P.R. China, Faculty of Laboratory Medicine, Center for Translational Medicine, North Sichuan Medical college, Nanchong, Sichuan, P.R. China

http://orcid.org/0000-0001-9283-7729

⨯
Yan Lei ,

Contributed equally to this work with: Qiang Wang, Yan Lei

Roles Data curation, Investigation, Methodology, Writing – original draft

Affiliations Department of Laboratory Medicine, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, P.R. China, Faculty of Laboratory Medicine, Center for Translational Medicine, North Sichuan Medical college, Nanchong, Sichuan, P.R. China
⨯
Xiaolan Lu,

Roles Data curation, Investigation, Methodology

Affiliation Department of Laboratory Medicine, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, P.R. China
⨯
Guangrong Wang,

Roles Data curation, Investigation, Methodology

Affiliations Department of Laboratory Medicine, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, P.R. China, Faculty of Laboratory Medicine, Center for Translational Medicine, North Sichuan Medical college, Nanchong, Sichuan, P.R. China
⨯
Qin Du,

Roles Data curation, Investigation, Methodology

Affiliation Department of Laboratory Medicine, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, P.R. China
⨯
Xiaolan Guo,

Roles Data curation, Investigation, Methodology

Affiliations Department of Laboratory Medicine, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, P.R. China, Faculty of Laboratory Medicine, Center for Translational Medicine, North Sichuan Medical college, Nanchong, Sichuan, P.R. China
⨯
Yan Xing,

Roles Data curation, Investigation

Affiliation Faculty of Laboratory Medicine, Center for Translational Medicine, North Sichuan Medical college, Nanchong, Sichuan, P.R. China
⨯
Guoyuan Zhang ,

Roles Conceptualization, Investigation

* E-mail: 13508099826@126.com (GZ); wangdongshengnc@163.com (DW)

Affiliation Department of Laboratory Medicine, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, P.R. China
⨯
Dongsheng Wang

Roles Conceptualization, Formal analysis, Supervision, Writing – review & editing

* E-mail: 13508099826@126.com (GZ); wangdongshengnc@163.com (DW)

Affiliation Department of Laboratory Medicine, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, P.R. China
⨯

Urea-mediated dissociation alleviate the false-positive Treponema pallidum-specific antibodies detected by ELISA

Qiang Wang,
Yan Lei,
Xiaolan Lu,
Guangrong Wang,
Qin Du,
Xiaolan Guo,
Yan Xing,
Guoyuan Zhang,
Dongsheng Wang

Published: March 5, 2019
https://doi.org/10.1371/journal.pone.0212893

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Treponema pallidum
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