Integrated routine workflow using next-generation sequencing and a fully-automated platform for the detection of KRAS, NRAS and BRAF mutations in formalin-fixed paraffin embedded samples with poor DNA quality in patients with colorectal carcinoma

Claire Franczak; Ludovic Dubouis; Pauline Gilson; Marie Husson; Marie Rouyer; Jessica Demange; Agnès Leroux; Jean-Louis Merlin; Alexandre Harlé

doi:10.1371/journal.pone.0212801

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Open Access

Peer-reviewed

Research Article

Integrated routine workflow using next-generation sequencing and a fully-automated platform for the detection of KRAS, NRAS and BRAF mutations in formalin-fixed paraffin embedded samples with poor DNA quality in patients with colorectal carcinoma

Claire Franczak,

Roles Writing – original draft

Affiliation Université de Lorraine, CNRS UMR 7039 CRAN, Institut de Cancérologie de Lorraine, Service de Biopathologie, Nancy, France
⨯
Ludovic Dubouis,

Roles Formal analysis, Writing – original draft

Affiliation Université de Lorraine, CNRS UMR 7039 CRAN, Institut de Cancérologie de Lorraine, Service de Biopathologie, Nancy, France
⨯
Pauline Gilson,

Roles Writing – review & editing

Affiliation Université de Lorraine, CNRS UMR 7039 CRAN, Institut de Cancérologie de Lorraine, Service de Biopathologie, Nancy, France
⨯
Marie Husson,

Roles Project administration, Supervision

Affiliation Institut de Cancérologie de Lorraine, Service de Biopathologie, Nancy, France
⨯
Marie Rouyer,

Roles Resources

Affiliation Institut de Cancérologie de Lorraine, Service de Biopathologie, Nancy, France
⨯
Jessica Demange,

Roles Resources

Affiliation Institut de Cancérologie de Lorraine, Service de Biopathologie, Nancy, France
⨯
Agnès Leroux,

Roles Resources

Affiliation Université de Lorraine, CNRS UMR 7039 CRAN, Institut de Cancérologie de Lorraine, Service de Biopathologie, Nancy, France
⨯
Jean-Louis Merlin,

Roles Supervision

Affiliation Université de Lorraine, CNRS UMR 7039 CRAN, Institut de Cancérologie de Lorraine, Service de Biopathologie, Nancy, France
⨯
Alexandre Harlé

Roles Supervision, Writing – review & editing

* E-mail: a.harle@nancy.unicancer.fr

Affiliation Université de Lorraine, CNRS UMR 7039 CRAN, Institut de Cancérologie de Lorraine, Service de Biopathologie, Nancy, France

http://orcid.org/0000-0001-9548-6522

⨯

Integrated routine workflow using next-generation sequencing and a fully-automated platform for the detection of KRAS, NRAS and BRAF mutations in formalin-fixed paraffin embedded samples with poor DNA quality in patients with colorectal carcinoma

Claire Franczak,
Ludovic Dubouis,
Pauline Gilson,
Marie Husson,
Marie Rouyer,
Jessica Demange,
Agnès Leroux,
Jean-Louis Merlin,
Alexandre Harlé

Published: February 27, 2019
https://doi.org/10.1371/journal.pone.0212801

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Mutation detection
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Next-generation sequencing
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Colorectal cancer
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DNA libraries
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DNA fragmentation
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DNA extraction
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DNA sequencing
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Mutation
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