Use of FFPE-derived DNA in next generation sequencing: DNA extraction methods

Samantha J. McDonough; Aditya Bhagwate; Zhifu Sun; Chen Wang; Michael Zschunke; Joshua A. Gorman; Karla J. Kopp; Julie M. Cunningham

doi:10.1371/journal.pone.0211400

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Open Access

Peer-reviewed

Research Article

Use of FFPE-derived DNA in next generation sequencing: DNA extraction methods

Samantha J. McDonough,

Roles Conceptualization, Methodology, Writing – original draft, Writing – review & editing

Affiliation Medical Genome Facility, Mayo Clinic, Rochester, Minnesota, United States of America
⨯
Aditya Bhagwate,

Roles Formal analysis, Writing – original draft, Writing – review & editing

Affiliation Department of Health Sciences Research, Division of Biomedical Statistics and Informatics, Mayo Clinic, Rochester, Minnesota, United States of America
⨯
Zhifu Sun,

Roles Formal analysis

Affiliation Department of Health Sciences Research, Division of Biomedical Statistics and Informatics, Mayo Clinic, Rochester, Minnesota, United States of America
⨯
Chen Wang,

Roles Formal analysis

Affiliation Department of Health Sciences Research, Division of Biomedical Statistics and Informatics, Mayo Clinic, Rochester, Minnesota, United States of America

http://orcid.org/0000-0003-2638-3081

⨯
Michael Zschunke,

Roles Methodology

Affiliation Pathology Research Core, Mayo Clinic, Rochester, Minnesota, United States of America
⨯
Joshua A. Gorman,

Roles Supervision

Affiliation Pathology Research Core, Mayo Clinic, Rochester, Minnesota, United States of America
⨯
Karla J. Kopp,

Roles Supervision

Affiliation Pathology Research Core, Mayo Clinic, Rochester, Minnesota, United States of America
⨯
Julie M. Cunningham

Roles Conceptualization, Project administration, Writing – original draft, Writing – review & editing

* E-mail: cunningham.julie@mayo.edu

Affiliations Medical Genome Facility, Mayo Clinic, Rochester, Minnesota, United States of America, Department of Laboratory Medicine & Pathology, Mayo Clinic, Rochester, Minnesota, United States of America

http://orcid.org/0000-0002-8159-3025

⨯

Use of FFPE-derived DNA in next generation sequencing: DNA extraction methods

Samantha J. McDonough,
Aditya Bhagwate,
Zhifu Sun,
Chen Wang,
Michael Zschunke,
Joshua A. Gorman,
Karla J. Kopp,
Julie M. Cunningham

Published: April 11, 2019
https://doi.org/10.1371/journal.pone.0211400

Reader Comments (3)

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1 Response

25 Mar 2020
14:41 GMT
Most Recent

SRA Project accession too long Posted by dlähnemann on 16 Mar 2020 at 12:53 GMT
0 Responses

25 Mar 2020
14:39 GMT
Most Recent

Data Availability Correction : PRJNA526013 Posted by abhagwate on 25 Mar 2020 at 14:39 GMT

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DNA extraction
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Next-generation sequencing
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DNA isolation
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Variant genotypes
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DNA libraries
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DNA sequencing
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