Discovery and characterization of potent IL-21 neutralizing antibodies via a novel alternating antigen immunization and humanization strategy

Reena Varkey; Qun Du; Jodi L. Karnell; Xiaodong Xiao; Kerry A. Casey; Rob Woods; Kim Rosenthal; Susan Wilson; William F. Dall’Acqua; Herren Wu; Ronald Herbst; Rachel Ettinger; Melissa Damschroder

doi:10.1371/journal.pone.0211236

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Open Access

Peer-reviewed

Research Article

Discovery and characterization of potent IL-21 neutralizing antibodies via a novel alternating antigen immunization and humanization strategy

Reena Varkey ,

Contributed equally to this work with: Reena Varkey, Qun Du

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Writing – original draft, Writing – review & editing

Affiliation Department of Antibody Discovery and Protein Engineering, MedImmune LLC, Gaithersburg, Maryland, United States of America
⨯
Qun Du ,

Contributed equally to this work with: Reena Varkey, Qun Du

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliation Department of Antibody Discovery and Protein Engineering, MedImmune LLC, Gaithersburg, Maryland, United States of America
⨯
Jodi L. Karnell,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

Current address: Viela Bio, Gaithersburg, Maryland, United States of America

Affiliation Department of Respiratory, Inflammation, and Autoimmunity, MedImmune LLC, Gaithersburg, Maryland, United States of America
⨯
Xiaodong Xiao,

Roles Conceptualization, Formal analysis, Supervision, Writing – original draft

Current address:Jecho Laboratories, Frederick, Maryland, United States of America

Affiliation Department of Antibody Discovery and Protein Engineering, MedImmune LLC, Gaithersburg, Maryland, United States of America
⨯
Kerry A. Casey,

Roles Conceptualization, Data curation, Formal analysis, Investigation

Affiliation Department of Respiratory, Inflammation, and Autoimmunity, MedImmune LLC, Gaithersburg, Maryland, United States of America
⨯
Rob Woods,

Roles Data curation, Formal analysis, Investigation

Current address: Novavax, Gaithersburg, Maryland, United States of America

Affiliation Department of Antibody Discovery and Protein Engineering, MedImmune LLC, Gaithersburg, Maryland, United States of America
⨯
Kim Rosenthal,

Roles Data curation, Formal analysis

Affiliation Department of Antibody Discovery and Protein Engineering, MedImmune LLC, Gaithersburg, Maryland, United States of America
⨯
Susan Wilson,

Roles Conceptualization, Data curation, Resources, Supervision

Affiliation Department of Antibody Discovery and Protein Engineering, MedImmune LLC, Gaithersburg, Maryland, United States of America
⨯
William F. Dall’Acqua,

Roles Resources, Supervision

Affiliation Department of Antibody Discovery and Protein Engineering, MedImmune LLC, Gaithersburg, Maryland, United States of America
⨯
Herren Wu,

Roles Project administration, Resources

Affiliation Department of Antibody Discovery and Protein Engineering, MedImmune LLC, Gaithersburg, Maryland, United States of America
⨯
Ronald Herbst,

Roles Project administration, Resources, Supervision

Affiliation Department of Respiratory, Inflammation, and Autoimmunity, MedImmune LLC, Gaithersburg, Maryland, United States of America
⨯
Rachel Ettinger,

Roles Conceptualization, Formal analysis, Methodology, Project administration, Resources, Validation, Writing – original draft, Writing – review & editing

Current address: Viela Bio, Gaithersburg, Maryland, United States of America

Affiliation Department of Respiratory, Inflammation, and Autoimmunity, MedImmune LLC, Gaithersburg, Maryland, United States of America
⨯
Melissa Damschroder

Roles Conceptualization, Formal analysis, Project administration, Resources, Supervision, Validation, Writing – review & editing

* E-mail: DamschroderM@medimmune.com

Affiliation Department of Antibody Discovery and Protein Engineering, MedImmune LLC, Gaithersburg, Maryland, United States of America

http://orcid.org/0000-0001-9453-6532

⨯

Discovery and characterization of potent IL-21 neutralizing antibodies via a novel alternating antigen immunization and humanization strategy

Reena Varkey,
Qun Du,
Jodi L. Karnell,
Xiaodong Xiao,
Kerry A. Casey,
Rob Woods,
Kim Rosenthal,
Susan Wilson,
William F. Dall’Acqua,
Herren Wu

Published: January 25, 2019
https://doi.org/10.1371/journal.pone.0211236

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