A new approach to Cas9-based genome editing in Aspergillus niger that is precise, efficient and selectable

• Laure M. C. Leynaud-Kieffer,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

  Affiliations Swiss Federal Institute of Technology Lausanne, Lausanne, Vaud, Switzerland, Joint Bioenergy Institute, Emeryville, CA, United States of America, Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, United States of America

  http://orcid.org/0000-0002-2074-9903

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• Samuel C. Curran,

  Roles Methodology, Supervision

  Affiliations Joint Bioenergy Institute, Emeryville, CA, United States of America, Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, United States of America, Comparative Biochemistry Graduate Group, University of California Berkeley, Berkeley, CA, United States of America

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• Irene Kim,

  Roles Methodology

  Affiliation Department of Chemistry, University of California, Berkeley, CA, United States of America

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• Jon K. Magnuson,

  Roles Supervision

  Affiliations Joint Bioenergy Institute, Emeryville, CA, United States of America, Chemical and Biological Process Development Group, Pacific Northwest National Laboratory, Richland, WA, United States of America

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• John M. Gladden,

  Roles Supervision

  Affiliations Joint Bioenergy Institute, Emeryville, CA, United States of America, Department of Biomass Science and Conversion Technology, Sandia National Laboratories, Livermore, CA, United States of America

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• Scott E. Baker,

  Roles Supervision

  Affiliations Joint Bioenergy Institute, Emeryville, CA, United States of America, Biosystems Design and Simulation Group, Environmental Molecular Sciences Division, Pacific Northwest National Laboratory, Richland, WA, United States of America

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• Blake A. Simmons

  Roles Project administration, Supervision, Validation, Writing – review & editing

  * E-mail: basimmons@lbl.gov

  Affiliations Joint Bioenergy Institute, Emeryville, CA, United States of America, Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, United States of America

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