Protein engineering strategies for improving the selective methylation of target CpG sites by a dCas9-directed cytosine methyltransferase in bacteria

• Tina Xiong,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Writing – original draft, Writing – review & editing

  Affiliation Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, United States of America

  ⨯
• Dahlia Rohm,

  Roles Data curation, Formal analysis, Investigation, Writing – review & editing

  Affiliation Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, United States of America

  ⨯
• Rachael E. Workman,

  Roles Investigation

  Affiliation Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland, United States of America

  ⨯
• Lauren Roundtree,

  Roles Investigation

  Affiliation Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, United States of America

  ⨯
• Carl D. Novina,

  Roles Funding acquisition, Writing – review & editing

  Affiliations Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Boston, Massachusetts, United States of America, Department of Medicine, Harvard Medical School, Boston, Massachusetts, United States of America, Broad Institute of Harvard and MIT, Cambridge, Massachusetts, United States of America

  ⨯
• Winston Timp,

  Roles Data curation, Formal analysis, Writing – review & editing

  Affiliation Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland, United States of America

  ⨯
• Marc Ostermeier

  Roles Conceptualization, Formal analysis, Funding acquisition, Writing – original draft, Writing – review & editing

  * E-mail: oster@jhu.edu

  Affiliation Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, United States of America

  http://orcid.org/0000-0003-0900-3665

  ⨯