Reducing phenolic off-flavors through CRISPR-based gene editing of the FDC1 gene in Saccharomyces cerevisiae x Saccharomyces eubayanus hybrid lager beer yeasts

Stijn Mertens; Brigida Gallone; Jan Steensels; Beatriz Herrera-Malaver; Jeroen Cortebeek; Robbe Nolmans; Veerle Saels; Valmik K. Vyas; Kevin J. Verstrepen

doi:10.1371/journal.pone.0209124

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Peer-reviewed

Research Article

Reducing phenolic off-flavors through CRISPR-based gene editing of the FDC1 gene in Saccharomyces cerevisiae x Saccharomyces eubayanus hybrid lager beer yeasts

Stijn Mertens,

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliations Laboratory for Genetics and Genomics, Centre of Microbial and Plant Genetics (CMPG), KU Leuven, Leuven, Belgium, Laboratory for Systems Biology, VIB Centre for Microbiology, Bio-Incubator, Leuven, Belgium, Leuven Institute for Beer Research, KU Leuven, Bio-Incubator, Leuven, Belgium
⨯
Brigida Gallone,

Roles Formal analysis, Investigation, Writing – original draft, Writing – review & editing

Affiliations Laboratory for Genetics and Genomics, Centre of Microbial and Plant Genetics (CMPG), KU Leuven, Leuven, Belgium, Laboratory for Systems Biology, VIB Centre for Microbiology, Bio-Incubator, Leuven, Belgium, Leuven Institute for Beer Research, KU Leuven, Bio-Incubator, Leuven, Belgium, Department of Plant Systems Biology, VIB, Gent, Belgium, Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium
⨯
Jan Steensels,

Roles Conceptualization, Writing – original draft, Writing – review & editing

Affiliations Laboratory for Genetics and Genomics, Centre of Microbial and Plant Genetics (CMPG), KU Leuven, Leuven, Belgium, Laboratory for Systems Biology, VIB Centre for Microbiology, Bio-Incubator, Leuven, Belgium, Leuven Institute for Beer Research, KU Leuven, Bio-Incubator, Leuven, Belgium
⨯
Beatriz Herrera-Malaver,

Roles Data curation

Affiliations Laboratory for Genetics and Genomics, Centre of Microbial and Plant Genetics (CMPG), KU Leuven, Leuven, Belgium, Laboratory for Systems Biology, VIB Centre for Microbiology, Bio-Incubator, Leuven, Belgium, Leuven Institute for Beer Research, KU Leuven, Bio-Incubator, Leuven, Belgium
⨯
Jeroen Cortebeek,

Roles Data curation, Investigation

Affiliations Laboratory for Genetics and Genomics, Centre of Microbial and Plant Genetics (CMPG), KU Leuven, Leuven, Belgium, Laboratory for Systems Biology, VIB Centre for Microbiology, Bio-Incubator, Leuven, Belgium, Leuven Institute for Beer Research, KU Leuven, Bio-Incubator, Leuven, Belgium
⨯
Robbe Nolmans,

Roles Investigation

Affiliations Laboratory for Genetics and Genomics, Centre of Microbial and Plant Genetics (CMPG), KU Leuven, Leuven, Belgium, Laboratory for Systems Biology, VIB Centre for Microbiology, Bio-Incubator, Leuven, Belgium, Leuven Institute for Beer Research, KU Leuven, Bio-Incubator, Leuven, Belgium
⨯
Veerle Saels,

Roles Investigation

Affiliations Laboratory for Genetics and Genomics, Centre of Microbial and Plant Genetics (CMPG), KU Leuven, Leuven, Belgium, Laboratory for Systems Biology, VIB Centre for Microbiology, Bio-Incubator, Leuven, Belgium, Leuven Institute for Beer Research, KU Leuven, Bio-Incubator, Leuven, Belgium
⨯
Valmik K. Vyas,

Roles Methodology

Affiliation Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, United States of America

http://orcid.org/0000-0003-2849-3399

⨯
Kevin J. Verstrepen

Roles Conceptualization, Funding acquisition, Supervision, Writing – review & editing

* E-mail: kevin.verstrepen@biw.vib-kuleuven.be

Affiliations Laboratory for Genetics and Genomics, Centre of Microbial and Plant Genetics (CMPG), KU Leuven, Leuven, Belgium, Laboratory for Systems Biology, VIB Centre for Microbiology, Bio-Incubator, Leuven, Belgium, Leuven Institute for Beer Research, KU Leuven, Bio-Incubator, Leuven, Belgium

http://orcid.org/0000-0002-3077-6219

⨯

Reducing phenolic off-flavors through CRISPR-based gene editing of the FDC1 gene in Saccharomyces cerevisiae x Saccharomyces eubayanus hybrid lager beer yeasts

Stijn Mertens,
Brigida Gallone,
Jan Steensels,
Beatriz Herrera-Malaver,
Jeroen Cortebeek,
Robbe Nolmans,
Veerle Saels,
Valmik K. Vyas,
Kevin J. Verstrepen

Published: January 9, 2019
https://doi.org/10.1371/journal.pone.0209124

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