Development and application of a Bacillus anthracis protective antigen domain-1 in-house ELISA for the detection of anti-protective antigen antibodies in cattle in Zambia

• Manyando Simbotwe,

  Roles Conceptualization, Data curation, Writing – original draft, Writing – review & editing

  Affiliations Division of Infection and Immunity, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan

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• Daisuke Fujikura,

  Roles Conceptualization, Methodology, Writing – review & editing

  Affiliations Division of Infection and Immunity, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan, Asahikawa Medical University Education Research Promotion Center, Asahikawa, Japan

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• Miyuki Ohnuma,

  Roles Investigation

  Affiliation Division of Infection and Immunity, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan

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• Ryosuke Omori,

  Roles Formal analysis

  Affiliations Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan, Division of Bioinformatics, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan

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• Yoshikazu Furuta,

  Roles Writing – review & editing

  Affiliations Division of Infection and Immunity, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan

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• Geoffrey Munkombwe Muuka,

  Roles Investigation

  Affiliation Bacteriology Section, Central Veterinary Research Institute, Ministry of Fisheries and Livestock, Lusaka, Zambia

  http://orcid.org/0000-0002-3494-0215

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• Bernard Mudenda Hang’ombe,

  Roles Conceptualization, Supervision

  Affiliation Microbiology Unit, School of Veterinary Medicine, University of Zambia, Lusaka, Zambia

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• Hideaki Higashi

  Roles Conceptualization, Investigation, Resources, Supervision, Writing – review & editing

  * E-mail: hidea-hi@czc.hokudai.ac.jp

  Affiliations Division of Infection and Immunity, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan, Hokudai Center for Zoonosis Control in Zambia, Lusaka, Zambia

  http://orcid.org/0000-0002-5370-7865

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