Comprehensive evaluation of candidate reference genes for real-time quantitative PCR (RT-qPCR) data normalization in nutri-cereal finger millet [Eleusine Coracana (L.)]

Palakolanu Sudhakar Reddy; Mahamaya G. Dhaware; Dumbala Srinivas Reddy; Bommineni Pradeep Reddy; Kummari Divya; Kiran K. Sharma; Pooja Bhatnagar-Mathur

doi:10.1371/journal.pone.0205668

Loading metrics

Open Access

Peer-reviewed

Research Article

Comprehensive evaluation of candidate reference genes for real-time quantitative PCR (RT-qPCR) data normalization in nutri-cereal finger millet [Eleusine Coracana (L.)]

Palakolanu Sudhakar Reddy ,

Roles Conceptualization, Data curation, Investigation, Supervision, Writing – original draft, Writing – review & editing

* E-mail: p.sudhakarreddy@cgiar.org

Affiliation International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad, Telangana, India

http://orcid.org/0000-0002-5341-187X

⨯
Mahamaya G. Dhaware,

Roles Data curation, Methodology, Validation

Affiliation International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad, Telangana, India
⨯
Dumbala Srinivas Reddy,

Roles Software, Validation, Writing – original draft

Affiliation International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad, Telangana, India
⨯
Bommineni Pradeep Reddy,

Roles Methodology

Affiliation International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad, Telangana, India
⨯
Kummari Divya,

Roles Methodology

Affiliation International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad, Telangana, India
⨯
Kiran K. Sharma,

Roles Formal analysis, Resources, Writing – review & editing

Affiliation International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad, Telangana, India
⨯
Pooja Bhatnagar-Mathur

Roles Conceptualization, Resources, Supervision, Writing – review & editing

Affiliation International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad, Telangana, India
⨯

Comprehensive evaluation of candidate reference genes for real-time quantitative PCR (RT-qPCR) data normalization in nutri-cereal finger millet [Eleusine Coracana (L.)]

Palakolanu Sudhakar Reddy,
Mahamaya G. Dhaware,
Dumbala Srinivas Reddy,
Bommineni Pradeep Reddy,
Kummari Divya,
Kiran K. Sharma,
Pooja Bhatnagar-Mathur

Published: October 15, 2018
https://doi.org/10.1371/journal.pone.0205668

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Millet
Is the Subject Area "Millet" applicable to this article?

Thanks for your feedback.
Gene expression
Is the Subject Area "Gene expression" applicable to this article?

Thanks for your feedback.
Cereal crops
Is the Subject Area "Cereal crops" applicable to this article?

Thanks for your feedback.
Polymerase chain reaction
Is the Subject Area "Polymerase chain reaction" applicable to this article?

Thanks for your feedback.
Drought adaptation
Is the Subject Area "Drought adaptation" applicable to this article?

Thanks for your feedback.
Plant resistance to abiotic stress
Is the Subject Area "Plant resistance to abiotic stress" applicable to this article?

Thanks for your feedback.
Leaves
Is the Subject Area "Leaves" applicable to this article?

Thanks for your feedback.
Crop genetics
Is the Subject Area "Crop genetics" applicable to this article?

Thanks for your feedback.