Eprobe mediated RT-qPCR for the detection of leukemia-associated fusion genes

Koji Tsuchiya; Yoko Tabe; Tomohiko Ai; Takahiro Ohkawa; Kengo Usui; Maiko Yuri; Shigeki Misawa; Soji Morishita; Tomoiku Takaku; Atsushi Kakimoto; Haeun Yang; Hiromichi Matsushita; Takeshi Hanami; Yasunari Yamanaka; Atsushi Okuzawa; Takashi Horii; Yoshihide Hayashizaki; Akimichi Ohsaka

doi:10.1371/journal.pone.0202429

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Open Access

Peer-reviewed

Research Article

Eprobe mediated RT-qPCR for the detection of leukemia-associated fusion genes

Koji Tsuchiya,

Roles Data curation, Formal analysis, Writing – original draft

Affiliations Division of Clinical Laboratory, Juntendo University Hospital, Tokyo, Japan, Department of Transfusion Medicine and Stem Cell Regulation, Graduate School of Medicine, Juntendo University, Tokyo, Japan
⨯
Yoko Tabe ,

Roles Conceptualization, Project administration, Supervision, Validation, Writing – review & editing

* E-mail: tabe@juntendo.ac.jp

Affiliations Department of Next Generation Hematology Laboratory Medicine, Graduate School of Medicine, Juntendo University, Tokyo, Japan, Department of Clinical Laboratory Medicine, Graduate School of Medicine, Juntendo University, Tokyo, Japan

http://orcid.org/0000-0002-3734-0346

⨯
Tomohiko Ai,

Roles Formal analysis, Writing – review & editing

Affiliation Department of Clinical Laboratory Medicine, Graduate School of Medicine, Juntendo University, Tokyo, Japan
⨯
Takahiro Ohkawa,

Roles Data curation, Formal analysis

Affiliation Nucleic Acid Diagnostic System Development Unit, Division of Genomic Technologies, RIKEN Center for Life Science Technologies, Kanagawa, Japan
⨯
Kengo Usui,

Roles Data curation, Formal analysis

Affiliations Nucleic Acid Diagnostic System Development Unit, Division of Genomic Technologies, RIKEN Center for Life Science Technologies, Kanagawa, Japan, Genetic Diagnosis Technology Unit, Division of Genomic Technologies, RIKEN Center for Life Science Technologies, Kanagawa, Japan
⨯
Maiko Yuri,

Roles Data curation

Affiliation Division of Clinical Laboratory, Juntendo University Hospital, Tokyo, Japan
⨯
Shigeki Misawa,

Roles Data curation, Formal analysis, Supervision

Affiliation Division of Clinical Laboratory, Juntendo University Hospital, Tokyo, Japan
⨯
Soji Morishita,

Roles Data curation, Formal analysis, Validation

Affiliation Department of Transfusion Medicine and Stem Cell Regulation, Graduate School of Medicine, Juntendo University, Tokyo, Japan
⨯
Tomoiku Takaku,

Roles Data curation, Formal analysis, Validation

Affiliation Department of Hematology, Juntendo University School of Medicine, Tokyo, Japan
⨯
Atsushi Kakimoto,

Roles Formal analysis, Investigation, Validation

Affiliation Department of Transfusion Medicine and Stem Cell Regulation, Graduate School of Medicine, Juntendo University, Tokyo, Japan
⨯
Haeun Yang,

Roles Data curation

Affiliation Department of Clinical Laboratory Medicine, Graduate School of Medicine, Juntendo University, Tokyo, Japan
⨯
Hiromichi Matsushita,

Roles Formal analysis, Writing – original draft

Affiliation Division of Pathology and Clinical Laboratories, National Cancer Center Hospital, Tokyo, Japan
⨯
Takeshi Hanami,

Roles Conceptualization, Formal analysis, Validation

Affiliation Genetic Diagnosis Technology Unit, Division of Genomic Technologies, RIKEN Center for Life Science Technologies, Kanagawa, Japan
⨯
Yasunari Yamanaka,

Roles Data curation, Formal analysis, Validation

Affiliation Preventive Medicine and Diagnosis Innovation Program, RIKEN, Wako, Japan
⨯
Atsushi Okuzawa,

Roles Data curation, Formal analysis

Affiliation Innovative Medical Technology Research & Development Center, Juntendo University School of Medicine, Tokyo, Japan
⨯
Takashi Horii,

Roles Formal analysis, Supervision

Affiliation Division of Clinical Laboratory, Juntendo University Hospital, Tokyo, Japan
⨯
Yoshihide Hayashizaki,

Roles Conceptualization, Formal analysis, Validation

Affiliation Preventive Medicine and Diagnosis Innovation Program, RIKEN, Wako, Japan
⨯
[ ... ],
Akimichi Ohsaka

Roles Conceptualization, Project administration, Supervision

Affiliations Division of Clinical Laboratory, Juntendo University Hospital, Tokyo, Japan, Department of Transfusion Medicine and Stem Cell Regulation, Graduate School of Medicine, Juntendo University, Tokyo, Japan, Department of Next Generation Hematology Laboratory Medicine, Graduate School of Medicine, Juntendo University, Tokyo, Japan
⨯
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Eprobe mediated RT-qPCR for the detection of leukemia-associated fusion genes

Koji Tsuchiya,
Yoko Tabe,
Tomohiko Ai,
Takahiro Ohkawa,
Kengo Usui,
Maiko Yuri,
Shigeki Misawa,
Soji Morishita,
Tomoiku Takaku,
Atsushi Kakimoto

Published: October 3, 2018
https://doi.org/10.1371/journal.pone.0202429

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Polymerase chain reaction
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Signal amplification
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Leukemia
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RNA amplification
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Fusion genes
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