Investigation of interactions between TLR2, MyD88 and TIRAP by bioluminescence resonance energy transfer is hampered by artefacts of protein overexpression

• Natália G. Sampaio ,

  Roles Conceptualization, Formal analysis, Investigation, Methodology, Project administration, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: Natalia.sampaio@rdm.ox.ac.uk

  Current address: MRC Human Immunology Unit, Radcliffe Department of Medicine, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United Kingdom

  Affiliations Population Health and Immunity Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia, Department of Medical Biology, University of Melbourne, Parkville, Victoria, Australia

  http://orcid.org/0000-0002-8396-1354

  ⨯
• Martina Kocan,

  Roles Methodology, Resources, Supervision

  Current address: The Florey Institute of Neuroscience and Mental Health, Parkville, Victoria, Australia

  Affiliation Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, Victoria, Australia

  ⨯
• Louis Schofield,

  Roles Conceptualization, Funding acquisition

  Affiliations Population Health and Immunity Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia, Australian Institute of Tropical Health and Medicine, James Cook University, Townsville, Queensland, Australia

  ⨯
• Kevin D. G. Pfleger,

  Roles Supervision, Writing – review & editing

  Affiliations Harry Perkins Institute of Medical Research and Centre for Medical Research, The University of Western Australia, Nedlands, Western Australia, Australia, Dimerix Limited, Nedlands, Western Australia, Australia

  ⨯
• Emily M. Eriksson

  Roles Supervision, Writing – review & editing

  Affiliations Population Health and Immunity Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia, Department of Medical Biology, University of Melbourne, Parkville, Victoria, Australia

  ⨯