Highly efficient scarless knock-in of reporter genes into human and mouse pluripotent stem cells via transient antibiotic selection

• Valentin M. Sluch ,

  Contributed equally to this work with: Valentin M. Sluch, Xitiz Chamling

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Supervision, Validation, Writing – original draft, Writing – review & editing

  Affiliation Department of Ophthalmology, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, United States of America

  http://orcid.org/0000-0003-3318-7986

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• Xitiz Chamling ,

  Contributed equally to this work with: Valentin M. Sluch, Xitiz Chamling

  Roles Conceptualization, Data curation, Investigation, Methodology, Resources, Supervision, Validation, Writing – review & editing

  Affiliation Department of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America

  http://orcid.org/0000-0001-6401-7715

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• Claire Wenger,

  Roles Resources, Validation, Writing – review & editing

  Affiliation McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine Baltimore, Maryland, United States of America

  http://orcid.org/0000-0002-0294-2291

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• Yukan Duan,

  Roles Resources, Writing – review & editing

  Affiliation Department of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America

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• Dennis S. Rice,

  Roles Supervision, Writing – review & editing

  Affiliation Department of Ophthalmology, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, United States of America

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• Donald J. Zack

  Roles Conceptualization, Formal analysis, Funding acquisition, Investigation, Supervision, Writing – review & editing

  * E-mail: dzack@jhmi.edu

  Affiliations Department of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America, McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine Baltimore, Maryland, United States of America, Department of Molecular Biology and Genetics, The Solomon H. Snyder Department of Neuroscience, Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America

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