An effective protocol for pharmacological defatting of primary human hepatocytes which is non-toxic to cholangiocytes or intrahepatic endothelial cells

Yuri L. Boteon; Lorraine Wallace; Amanda P. C. S. Boteon; Darius F. Mirza; Hynek Mergental; Ricky H. Bhogal; Simon Afford

doi:10.1371/journal.pone.0201419

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Open Access

Peer-reviewed

Research Article

An effective protocol for pharmacological defatting of primary human hepatocytes which is non-toxic to cholangiocytes or intrahepatic endothelial cells

Yuri L. Boteon,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing – original draft, Writing – review & editing

Affiliations Liver Unit, Queen Elizabeth Hospital, University Hospitals Birmingham NHS Foundation Trust, Birmingham, United Kingdom, National Institute for Health Research (NIHR) Birmingham Biomedical Research Centre, Institute of Immunology and Immunotherapy, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom

http://orcid.org/0000-0002-1709-9284

⨯
Lorraine Wallace,

Roles Investigation, Methodology, Writing – review & editing

Affiliation National Institute for Health Research (NIHR) Birmingham Biomedical Research Centre, Institute of Immunology and Immunotherapy, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom
⨯
Amanda P. C. S. Boteon,

Roles Investigation, Writing – original draft

Affiliation Liver Unit, Queen Elizabeth Hospital, University Hospitals Birmingham NHS Foundation Trust, Birmingham, United Kingdom
⨯
Darius F. Mirza,

Roles Conceptualization, Writing – review & editing

Affiliation Liver Unit, Queen Elizabeth Hospital, University Hospitals Birmingham NHS Foundation Trust, Birmingham, United Kingdom
⨯
Hynek Mergental,

Roles Writing – review & editing

Affiliation Liver Unit, Queen Elizabeth Hospital, University Hospitals Birmingham NHS Foundation Trust, Birmingham, United Kingdom
⨯
Ricky H. Bhogal,

Roles Conceptualization, Methodology, Supervision, Writing – review & editing

Affiliation National Institute for Health Research (NIHR) Birmingham Biomedical Research Centre, Institute of Immunology and Immunotherapy, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom
⨯
Simon Afford

Roles Conceptualization, Methodology, Project administration, Resources, Supervision, Writing – review & editing

* E-mail: s.c.afford@bham.ac.uk

Affiliation National Institute for Health Research (NIHR) Birmingham Biomedical Research Centre, Institute of Immunology and Immunotherapy, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom
⨯

An effective protocol for pharmacological defatting of primary human hepatocytes which is non-toxic to cholangiocytes or intrahepatic endothelial cells

Yuri L. Boteon,
Lorraine Wallace,
Amanda P. C. S. Boteon,
Darius F. Mirza,
Hynek Mergental,
Ricky H. Bhogal,
Simon Afford

Published: July 25, 2018
https://doi.org/10.1371/journal.pone.0201419

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