Fast Temperature-Gradient COLD PCR for the enrichment of the paternally inherited SNPs in cell free fetal DNA; an application to non-invasive prenatal diagnosis of β-thalassaemia

• Stefania Byrou,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft

  Affiliations Molecular Genetics Thalassaemia Department, The Cyprus Institute of Neurology and Genetics, Nicosia, Cyprus, The Cyprus School of Molecular Medicine, Nicosia, Cyprus

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• G. Mike Makrigiorgos,

  Roles Conceptualization, Methodology, Writing – review & editing

  Affiliation Department of Radiation Oncology, Division of Medical Physics & Biophysics, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts, United States of America

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• Agathoklis Christofides,

  Roles Resources, Writing – review & editing

  Affiliation Medical Centre Fetal Medicine Department, Archbishop Makarios III Hospital, Nicosia, Cyprus

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• Ioannis Kallikas,

  Roles Resources, Writing – review & editing

  Affiliation Ultrasound and fetal medicine centre, Nicosia, Cyprus

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• Thessalia Papasavva ,

  Contributed equally to this work with: Thessalia Papasavva, Marina Kleanthous

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Methodology, Project administration, Resources, Supervision, Writing – original draft, Writing – review & editing

  * E-mail: thesalia@cing.ac.cy

  ¶‡ TP and MK are joint senior authors on this work.

  Affiliations Molecular Genetics Thalassaemia Department, The Cyprus Institute of Neurology and Genetics, Nicosia, Cyprus, The Cyprus School of Molecular Medicine, Nicosia, Cyprus

  http://orcid.org/0000-0001-9279-4810

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• Marina Kleanthous

  Contributed equally to this work with: Thessalia Papasavva, Marina Kleanthous

  Roles Conceptualization, Data curation, Funding acquisition, Project administration, Resources, Supervision, Writing – review & editing

  ¶‡ TP and MK are joint senior authors on this work.

  Affiliations Molecular Genetics Thalassaemia Department, The Cyprus Institute of Neurology and Genetics, Nicosia, Cyprus, The Cyprus School of Molecular Medicine, Nicosia, Cyprus

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