Structure-guided approach to site-specific fluorophore labeling of the lac repressor LacI

Kalle Kipper; Nadja Eremina; Emil Marklund; Sumera Tubasum; Guanzhong Mao; Laura Christina Lehmann; Johan Elf; Sebastian Deindl

doi:10.1371/journal.pone.0198416

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Open Access

Peer-reviewed

Research Article

Structure-guided approach to site-specific fluorophore labeling of the lac repressor LacI

Kalle Kipper ,

Contributed equally to this work with: Kalle Kipper, Nadja Eremina, Emil Marklund

Roles Conceptualization, Data curation, Investigation, Writing – original draft, Writing – review & editing

Affiliation Department of Cell and Molecular Biology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden
⨯
Nadja Eremina ,

Contributed equally to this work with: Kalle Kipper, Nadja Eremina, Emil Marklund

Roles Data curation, Investigation, Validation, Writing – review & editing

Affiliation Department of Cell and Molecular Biology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden
⨯
Emil Marklund ,

Contributed equally to this work with: Kalle Kipper, Nadja Eremina, Emil Marklund

Roles Conceptualization, Methodology, Writing – review & editing

Affiliation Department of Cell and Molecular Biology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden
⨯
Sumera Tubasum,

Roles Investigation, Writing – review & editing

Affiliation Department of Cell and Molecular Biology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden
⨯
Guanzhong Mao,

Roles Formal analysis, Investigation

Affiliation Department of Cell and Molecular Biology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden
⨯
Laura Christina Lehmann,

Roles Investigation, Visualization, Writing – review & editing

Affiliation Department of Cell and Molecular Biology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden
⨯
Johan Elf,

Roles Conceptualization, Supervision, Writing – review & editing

Affiliation Department of Cell and Molecular Biology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden
⨯
Sebastian Deindl

Roles Conceptualization, Funding acquisition, Methodology, Supervision, Visualization, Writing – original draft, Writing – review & editing

* E-mail: sebastian.deindl@icm.uu.se

Affiliation Department of Cell and Molecular Biology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden

http://orcid.org/0000-0001-6807-8654

⨯

Structure-guided approach to site-specific fluorophore labeling of the lac repressor LacI

Kalle Kipper,
Nadja Eremina,
Emil Marklund,
Sumera Tubasum,
Guanzhong Mao,
Laura Christina Lehmann,
Johan Elf,
Sebastian Deindl

Published: June 1, 2018
https://doi.org/10.1371/journal.pone.0198416

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Cysteine
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Dimerization
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Fluorescence imaging
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DNA-binding proteins
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