High-throughput evaluation of T7 promoter variants using biased randomization and DNA barcoding

Ryo Komura; Wataru Aoki; Keisuke Motone; Atsushi Satomura; Mitsuyoshi Ueda

doi:10.1371/journal.pone.0196905

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Open Access

Peer-reviewed

Research Article

High-throughput evaluation of T7 promoter variants using biased randomization and DNA barcoding

Ryo Komura,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliation Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto, Japan
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Wataru Aoki,

Roles Conceptualization, Data curation, Methodology, Validation, Writing – original draft, Writing – review & editing

Affiliation Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto, Japan
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Keisuke Motone,

Roles Formal analysis, Methodology, Software, Writing – review & editing

Affiliations Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto, Japan, Japan Society for the Promotion of Science, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto, Japan
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Atsushi Satomura,

Roles Conceptualization, Methodology, Writing – review & editing

Affiliations Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto, Japan, Japan Society for the Promotion of Science, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto, Japan
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Mitsuyoshi Ueda

Roles Supervision, Writing – review & editing

* E-mail: miueda@kais.kyoto-u.ac.jp

Affiliation Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto, Japan

http://orcid.org/0000-0003-2268-2391

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High-throughput evaluation of T7 promoter variants using biased randomization and DNA barcoding

Ryo Komura,
Wataru Aoki,
Keisuke Motone,
Atsushi Satomura,
Mitsuyoshi Ueda

Published: May 7, 2018
https://doi.org/10.1371/journal.pone.0196905

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DNA transcription
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Polymerase chain reaction
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Gene expression
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cDNA libraries
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