An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes

• Simon E. Tröder,

  Roles Conceptualization, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

  Affiliations Cologne Excellence Cluster for Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, Germany, in vivo Research Facility, Medical Faculty, University of Cologne, Cologne, Germany

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• Lena K. Ebert,

  Roles Investigation, Resources

  Affiliations Cologne Excellence Cluster for Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, Germany, Department II of Internal Medicine and Center for Molecular Medicine Cologne (CMMC), University of Cologne, Cologne, Germany

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• Linus Butt,

  Roles Investigation, Resources

  Affiliations Cologne Excellence Cluster for Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, Germany, Department II of Internal Medicine and Center for Molecular Medicine Cologne (CMMC), University of Cologne, Cologne, Germany

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• Sonja Assenmacher,

  Roles Investigation

  Affiliations Cologne Excellence Cluster for Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, Germany, in vivo Research Facility, Medical Faculty, University of Cologne, Cologne, Germany

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• Bernhard Schermer,

  Roles Funding acquisition, Resources

  Affiliations Cologne Excellence Cluster for Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, Germany, Department II of Internal Medicine and Center for Molecular Medicine Cologne (CMMC), University of Cologne, Cologne, Germany, Systems Biology of Aging Cologne (Sybacol), University of Cologne, Cologne, Germany

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• Branko Zevnik

  Roles Conceptualization, Funding acquisition, Methodology, Validation, Writing – original draft, Writing – review & editing

  * E-mail: branko.zevnik@uk-koeln.de

  Affiliations Cologne Excellence Cluster for Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, Germany, in vivo Research Facility, Medical Faculty, University of Cologne, Cologne, Germany

  http://orcid.org/0000-0001-7845-9522

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